000			04105nam a22003615i 4500
001			232990
003			ES-VaUE
005			20221220020431.0
007			cr nn 008mamaa
008			180531s2018 xxu| s |||| 0|eng d
020			_a9781493978779
024	7		_a10.1007/978-1-4939-7877-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCellulases _bMethods and Protocols _cedited by Mette Lübeck.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIII, 324 páginas) _b58 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1796
505	0		_aCellulases: Role in Lignocellulosic Biomass Utilization -- Discovery of a Novel Fungus with an Extraordinary b-Glucosidase -- Discovery of New Cellulases Using Classical or Molecular Methods -- Isolation and Screening of Cellulolytic Filamentous Fungi -- Isolation and Screening of Cellulose Degrading Microorganisms from Different Ecological Niches -- Isolation of Cellulolytic Bacteria from the Rumen -- Methods for Discovery of Novel Cellulosomal Cellulases using Genomics and Biochemical Tools -- Metatranscriptomic Techniques for Identifying Cellulases in Termites and Their Symbionts -- Discovery of Novel Cellulases Using Proteomic Strategies -- Identification of Key Components for the Optimization of Cellulase Mixtures Using a Proteomic Strategy -- Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus niger -- Advanced Cloning Tools for Construction of Designer Cellulosomes -- Evaluation of Thermal Stability of Cellulosomal Hydrolases and Their complex Formation -- Endoglucanase (EG) Activity Assays -- Cellobiohydolase (CBH) Activity Assays -- Two-Dimensional High-Throughput Endo-Enzyme Screening Assays Based on Chromogenic Polysaccharide Hydrogel and Complex Biomass Substrates -- Analytical Tools for Characterizing Cellulose-Active Lytic Polysaccharide Monooxygenases (LPMOs) -- Light Induced Electron Transfer Protocol for Enzymatic Oxidation of Polysaccharide -- Purification of Cellulase from Cellulolytic Microorganisms and Characterization of the Total Cellulase Activities (TCA) -- Fungal On-Site Production of Cellulolytic Enzymes by the Sequential Cultivation Method -- Test of Efficacy of Cellulases for Biomass Degradation -- Homology Modeling for Enzyme Design.
520			_aThis volume discusses the latest research and techniques used to study novel cellulases. The chapters in this book explore topics such as the role of cellulases in lignocellulose deconstruction for generation of a sugar platform for biofuel; screening for novel fungal b-glucosidases in a variety of fungal strains; discovery of novel enzymes in bacteria or fungi; protocols for isolating cellulolytic rumen bacteria; analysis of enzyme mixtures produced by ligocellulolytic fungi; and homology modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cellulases: Methods and Protocols is a valuable resource for biochemists, molecular biologists, chemical engineers, and industrial researchers interested in this field.
700	1		_aLübeck, Mette _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493978762
776	0	8	_iPrinted edition: _z9781493978786
776	0	8	_iPrinted edition: _z9781493993123
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7877-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c232990 _d232990