000			04288nam a22003855i 4500
001			233000
003			ES-VaUE
005			20221220020432.0
007			cr nn 008mamaa
008			190829s2020 xxu| s |||| 0|eng d
020			_a9781493997404
024	7		_a10.1007/978-1-4939-9740-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aElectroporation Protocols _bMicroorganism, Mammalian System, and Nanodevice _cedited by Shulin Li, Lingqian Chang, Justin Teissie.
250			_a3rd edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 196 páginas) _b38 ilustraciones, 31 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2050
505	0		_aCell Size-specific Transfection by Micropillar Array Electroporation -- Soft Electroporation through 3D Hollow Nanoelectrodes -- Micromachining of Polymeric Microfluidic Micro-/Nano-Electroporation Device -- 3D Nanochannel Array for High-Throughput Cell Manipulation and Electroporation -- Low-Voltage Flow-Through Electroporation Membrane and Method -- Nanofountain Probe Electroporation for Monoclonal Cell Line Generation -- 3D Nanochannel Electroporation for Macromolecular Nucleotides Delivery -- Nanoelectroporation and Collection of Genetically Modified Exosomes in Primary Cultures of Dendritic Cells -- Electroporation of CRISPR-Cas9 into Malignant B Cells for Loss-of-function Studies of Target Gene via Knockout -- Microfluidic Device for Localized Electroporation -- Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation -- Targeted in vivo Electroporation Using Nanoengineered Microelectrodes -- Zygote Electroporation for CRISPR/Cas9 Delivery to Generate Genetically Modified Mice -- Genome Editing in Staphylococcus aureus by Conditional Recombineering and CRISPR/Cas9-mediated Counterselection -- Tissue Nano-Transfection of Surgically Exposed Stroma -- Transformation of a Model Microalga, Chlamydomonas reinhardtii without Cell-wall Removal -- Electroporation Transformation Protocol for Phaeodactylum tricornutum -- Efficient Transformation of the Diatoms Phaeodactylum tricornutum by Multi-pulse Electroporation -- Rapid and Effective Electroporation Protocol for Nannochloropsis oceanica -- Electroporation Procedures for Genetic Modification of Green Algae Chlorella spp. -- Electrotransformation of Saccharomyces cerevisiae. .
520			_aThis third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice, Third Edition aims to be an invaluable resource for investigators both in and outside of this field. .
700	1		_aLi, Shulin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aChang, Lingqian _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTeissie, Justin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493997398
776	0	8	_iPrinted edition: _z9781493997411
776	0	8	_iPrinted edition: _z9781493997428
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9740-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233000 _d233000