000			03655nam a22003615i 4500
001			233005
003			ES-VaUE
005			20221220020432.0
007			cr nn 008mamaa
008			191001s2020 xxu| s |||| 0|eng d
020			_a9781493998456
024	7		_a10.1007/978-1-4939-9845-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSnake and Spider Toxins _bMethods and Protocols _cedited by Avi Priel.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (X, 293 páginas) _b46 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2068
505	0		_aSnake- and Spider-Venom-Derived Toxins as Lead Compounds for Drug Development -- Analytics for Bioactivity Profiling of Complex Mixtures with a Focus on Venoms -- Venom Collection from Spiders and Snakes: Voluntary and Involuntary Extractions ("Milking") and Venom Gland Extractions -- Production and Purification of Recombinant Toxins -- RNA-Sequencing of Snake Venom Glands -- Exploring Toxin Evolution: Venom Protein Transcript Sequencing and Transcriptome-Guided High-Throughput Proteomics -- Three-Dimensional Structure Determination of Peptides using Solution Nuclear Magnetic Resonance Spectroscopy -- Determining the Structures of the Snake and Spider Toxins by X-Rays -- MALDI-TOF Mass Spectrometric Profiling of Spider Venoms -- Methods for Evaluation of a Snake Venom Derived Disintegrin in Animal Models of Human Cancer -- Cell-Based Adhesion Assays for Isolation of Snake Venom's Integrin Antagonists -- Using C. elegans to Study the Effects of Toxins in Sensory Ion Channels In Vivo -- Measurements of Cell Death Induced by Snake and Spider's Venoms and Derived Toxins -- Using Toxins in Brain Slice Recordings -- High-Throughput Calcium Imaging Screen of Toxins' Function in Dissociated Sensory Neurons -- Synthesizing and Expressing Native Ion Channels.
520			_aThis volume explores techniques and protocols, across various biological disciplines, used to study snake and spider peptide toxins. The chapters in this book are organized into four parts and cover topics such as the use of toxins in drug development; analysis of bioactivity of complex mixtures like venom; extraction of venom glands and the production of toxins; characterization of toxins from the RNA level to the peptide structure; and the determination of the toxin's biological function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Snake and Spider Toxins: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field. .
700	1		_aPriel, Avi _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493998449
776	0	8	_iPrinted edition: _z9781493998463
776	0	8	_iPrinted edition: _z9781493998470
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9845-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233005 _d233005