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020 _a9781493998692
024 7 _a10.1007/978-1-4939-9869-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aProtein Nanotechnology
_bProtocols, Instrumentation, and Applications
_cedited by Juliet A. Gerrard, Laura J. Domigan.
250 _a3rd edition 2020
264 1 _aNew York, NY
_bSpringer International Publising
_c2020
300 _a1 recurso en línea (XIII, 331 páginas)
_b84 ilustraciones, 64 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2073
505 0 _aIntroduction to Protein Nanotechnology -- Protein Expression in the Baculovirus-Insect Cell Expression System -- Peroxiredoxin Proteins as Building Blocks for Nanotechnology -- Formation of Amphipathic Monolayers from Fungal Hydrophobin Proteins -- ATP Synthase: Expression, Purification, and Function -- Molecular Superglues: Discovery and Engineering Orthogonalization -- Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes -- Adding Function to Protein Scaffolds -- Virus-Derived Nanoparticles -- Strategies for Increasing Protein Stability -- Generation of High Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol -- Nanotechnology with S-Layer Proteins -- Preparation of Proteins and Macromolecular Assemblies for Cryo-Electron Microscopy -- Atomic Force Microscopy of Proteins -- Native Protein Mass Spectrometry -- Nanoparticle Tracking Analysis of b-Casein Nanocarriers -- Molecular Dynamics Simulation of Proteins.
520 _aThis third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation, and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field. .
700 1 _aGerrard, Juliet A
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aDomigan, Laura J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493998685
776 0 8 _iPrinted edition:
_z9781493998708
776 0 8 _iPrinted edition:
_z9781493998715
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9869-2
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2020
999 _c233007
_d233007