000			03811nam a22003735i 4500
001			233010
003			ES-VaUE
005			20221220020433.0
007			cr nn 008mamaa
008			191114s2020 xxu| s |||| 0|eng d
020			_a9781493999040
024	7		_a10.1007/978-1-4939-9904-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aChimeric RNA _bMethods and Protocols _cedited by Hui Li, Justin Elfman.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XII, 262 páginas) _b56 ilustraciones, 52 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2079
505	0		_aPrediction, Characterization, and In Silico Validation of Chimeric RNAs -- Identification of Fusion Transcripts from Unaligned RNA-Seq Reads Using ChimeRScope -- Identification of Chimeric RNAs Using RNA-Seq Reads and Protein-Protein Interactions of Translated Chimeras -- Gene Fusion Discovery with INTEGRATE -- Case Study: Systematic Detection and Prioritization of Gene Fusions in Cancer by RNA-Seq: A DIY Toolkit -- Detection and Measurement of Chimeric RNAs by RT-PCR -- Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cells -- RNase Protection Assay -- Validation of Chimeric Fusion Peptides Using Proteomics Data -- NanoString nCounter Technology: High-Throughput RNA Validation -- Knockdown of Chimeric RNA by RNAi -- Overexpression of Chimeric RNA by Retroviral Transduction -- Separation of Nuclear and Cytoplasmic Fractions for Chimeric RNA Characterization -- Confirmation of Transcriptional Read-Through Events by RT-PCR -- Validating Gene Fusion as the Source of Chimeric RNAs -- Chimeric RNA and Exosomes-Based Liquid Biopsy -- SMaRT for Therapeutic Purposes -- Case Study: Landscape of Chimeric RNAs in Bladder Cancer -- Case Study: The Recurrent Fusion RNA DUS4L-BCAP29 in Non-Cancer Human Tissues and Cells.
520			_aThis book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinformatics tools, the book continues with experimental validation using RT-PCR, hybridization methods for RNA, and the use of mass spectrometry data for fusion protein validation, as well as functional and mechanistic studies of chimeric RNAs and translational applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chimeric RNA: Methods and Protocols is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome.
700	1		_aLi, Hui _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aElfman, Justin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493999033
776	0	8	_iPrinted edition: _z9781493999057
776	0	8	_iPrinted edition: _z9781493999064
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9904-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233010 _d233010