000			04640nam a22003735i 4500
001			233011
003			ES-VaUE
005			20221220020433.0
007			cr nn 008mamaa
008			191125s2020 xxu| s |||| 0|eng d
020			_a9781493998227
024	7		_a10.1007/978-1-4939-9822-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Eukaryotic RNA Exosome _bMethods and Protocols _cedited by John LaCava, Štěpánka Vaňáčová.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publishing _c2020
300			_a1 recurso en línea (XIII, 517 páginas) _b79 ilustraciones, 52 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2062
505	0		_aThe RNA Exosome and Human Disease -- The Bacterial Counterparts of the Eukaryotic Exosome: An Evolutionary Perspective -- In vitro Characterization of the Prokaryotic Counterparts of the Exosome Complex -- Enzymatic Analysis of Reconstituted Archaeal Exosomes -- Protocols for Northern Analysis of Exosome Substrates and Other Non-coding RNAs -- Mapping Exosome-substrate Interactions in vivo by UV-crosslinking -- Global Identification of Human Exosome Substrates using RNA Interference and RNA-sequencing -- High-resolution Mapping of 3' Extremities of RNA Exosome Substrates by 3' RACE-seq -- Determining mRNA Stability by Metabolic RNA Labeling and Chemical Nucleoside Conversion.-Thiouridine-to-Cytidine Conversion Sequencing (TUC-seq) to Measure mRNA Transcription and Degradation Rates -- RNA Exosomes and their Co-factors -- Purification of Endogenous Tagged TRAMP4/5 and Exosome Complexes from Yeast and in vitro Polyadenylation-Exosome Activation Assays -- Comparative poly(A)+ RNA Interactome Capture of RNA Surveillance Mutants -- Purification and in vitro Analysis of the Exosome Cofactors Nrd1-Nab3 and Trf4-Air2 -- Affinity Proteomic Analysis of the Human Exosome and its Cofactor Complexes -- In vitro Characterization of the Activity of the Mammalian RNA exosome on mRNAs in Ribosomal Translation Complexes -- Native Mass Spectrometry Analysis of Affinity-captured Endogenous Yeast RNA Exosome Complexes -- Chemical Cross-Linking and Mass Spectrometric Analysis of the Endogenous Yeast Exosome Complexes -- Cryo-Electron Microscopy of Endogenous Yeast Exosomes -- Strategies for Generating RNA Exosome Complexes from Recombinant Expression Hosts -- Reconstitution of S. cerevisiae RNA Exosome Complexes Using Recombinantly Expressed Proteins -- Reconstitution of the Schizosaccharomyces pombe RNA Exosome -- Reconstitution of the Human Nuclear RNA Exosome -- Purification and Reconstitution of the S. cerevisiae TRAMP and Ski Complexes for Biochemical and Structural Studies. .
520			_aThis volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms. Chapters guide readers through methods that e.g. delineate eukaryotic exosomes' origins in prokaryotes, probe its RNA substrates, adapter complexes, and macromolecular interaction of networks, and establish critical structure-function relationships. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Eukaryotic RNA Exosome: Methods and Protocols aims to ensure successful results in the further study of this vital field. Authoritative and cutting-edge, Th e Eukaryotic RNA Exosome: Methods and Protocols aims to ensure successful results in the further study of this vital field. Chapters 5 and 6 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
700	1		_aLaCava, John _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aVaňáčová, Štěpánka _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493998210
776	0	8	_iPrinted edition: _z9781493998234
776	0	8	_iPrinted edition: _z9781493998241
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9822-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233011 _d233011