000			03744nam a22003735i 4500
001			233018
003			ES-VaUE
005			20221220020433.0
007			cr nn 008mamaa
008			200127s2020 xxu| s |||| 0|eng d
020			_a9781071602591
024	7		_a10.1007/978-1-0716-0259-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aImmunity in Insects _cedited by Federica Sandrelli, Gianluca Tettamanti.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 246 páginas) _b50 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aCharacterization of Insect Immune Systems from Genomic Data -- Analyzing Immunity in Non-model Insects using de novo Transcriptomics -- Preparation of Insect Protein Samples for Label-free Proteomic Quantification by LC-mass Spectrometry -- Quantification of Blood Cells in Drosophila melanogaster and other Insects -- Methods to Quantify in vivo Phagocytic Uptake and Opsonization of Live or Killed microbes in Drosophila melanogaster -- Analysis of Cellular Immune Responses in Lepidopteran Larvae -- Detection of Enzyme Distribution, Expression, Activation, and Activity of Insect Prophenoloxidase -- Basic Methods to Evaluate Humoral Immunity Processes in Lepidoptera larvae -- Methods for the in vitro Examination of the Antibacterial and Cytotoxic Activities of Antimicrobial Peptides -- Characterization of Antimicrobial Peptide - Membrane Interaction Using All-atom Molecular Dynamic Simulation -- Manipulating the Mosquito Microbiota to Study its Function -- Discovery and Analysis of RNA Viruses in Insects -- A Workflow for Infection of Drosophila with Entomopathogenic Nematodes to Monitor Immune Gene Transcriptional Activity -- Oral Infection in a Germ-Free Bombyx mori Model -- Silkworm Infection Model for Evaluating Pathogen Virulence. .
520			_aThis volume details methods and protocols necessary to further the study of insect immunity. Chapters guide readers through up-to-date genomic and transcriptomic approaches, insect samples for proteomic analysis, hemocytes in Drosophila, cellular response in Lepidoptera, insect AMPs, manipulate the composition of mosquito microbiota, viral infections in insects, infections by entomopathogenic nematodes, immune response following oral infections, and protocols to to monitor the effect of septic infections with human pathogens using B. mori as a model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunity in Insects aims to ensure successful results in the further study of this vital field.Incl .
700	1		_aSandrelli, Federica _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTettamanti, Gianluca _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071602584
776	0	8	_iPrinted edition: _z9781071602607
776	0	8	_iPrinted edition: _z9781071602614
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0259-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233018 _d233018