000			04042nam a22003615i 4500
001			233019
003			ES-VaUE
005			20221220020433.0
007			cr nn 008mamaa
008			200127s2020 xxu| s |||| 0|eng d
020			_a9781071603239
024	7		_a10.1007/978-1-0716-0323-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDNA Electrophoresis _bMethods and Protocols _cedited by Katsuhiro Hanada.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (X, 228 páginas) _b70 ilustraciones, 40 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2119
505	0		_a Introduction and Perspectives of DNA Electrophoresis -- Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers -- Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage -- 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast -- Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures -- Alkali Comet Assay in Genotoxicity Tests -- The Analysis of DNA Interstrand Crosslinks and their Repair by the Modified Comet Assay -- Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis -- Detection of DNA Damage-Induced DSBs by the Contour-Clamped Homogeneous Electric Field (CHEF) System in Mammalian Cells -- Investigation of DNA Double-Strand Breaks Induced in Host Cells following Infection with Genotoxic Bacteria -- Monitoring of DNA Replication and DNA Double-Strand Breaks in Saccharomyces cerevisiae by Pulsed-Field Gel Electrophoresis (PFGE) -- Pulsed-Field Gel Electrophoresis for Detecting Chromosomal DNA Breakage in Fission Yeast -- Detection of DNA Double-Strand Breaks by Pulsed-Field Gel Electrophoresis of Circular Bacterial Chromosomes -- Detection of Bleomycin-Induced DNA Double-Strand Breaks in Escherichia coli by Pulsed-Field Gel Electrophoresis using a Rotating Gel Electrophoresis System -- Circle-Seq: Isolation and Sequencing of Chromosome-Derived Circular DNA Elements in Cells -- Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation -- DNA Fragments Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP) -- Post-Labeling/PAGE Method for Detection of DNA Adducts.
520			_aThis volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field.
700	1		_aHanada, Katsuhiro _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071603222
776	0	8	_iPrinted edition: _z9781071603246
776	0	8	_iPrinted edition: _z9781071603253
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0323-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233019 _d233019