000			03923nam a22003615i 4500
001			233020
003			ES-VaUE
005			20221220020433.0
007			cr nn 008mamaa
008			200130s2020 xxu| s |||| 0|eng d
020			_a9781071602553
024	7		_a10.1007/978-1-0716-0255-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aXenotransplantation _bMethods and Protocols _cedited by Cristina Costa.
250			_a2nd edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 363 páginas) _b65 ilustraciones, 49 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2110
505	0		_aIntroduction: The Present Status of Xenotransplantation Research -- Tools for Molecular Studies in Xenotransplantation -- Proteomic Protocols for Differential Protein Expression Analyses -- Specific Detection of Neu5Gc in Animal Tissues by Immunohistochemistry -- Xenoantibodies and Complement Activity Determinations by Flow Cytometry in Pig-to-Primate Xenotrasplantation -- 3D Cell-Culture Models for the Assessment of Anticoagulant and Anti-inflammatory Properties of Endothelial Cells -- Cell-based Assays for Modeling Xenogeneic Immune Responses -- Determination of Redox Status in Serum -- Generation of Genetically Modified Mice using CRISPR/Cas9 -- Genome-wide PERV Inactivation in Pigs using CRISPR cas9 -- Co-transplantation of Vascularized Thymic Graft with Kidney in Pig-to-Non-human Primates for the Induction of Tolerance Across Xenogeneic Barriers -- Xenogeneic Lung Transplantation Models -- Liver Xenotransplantation in a Non-human Primate Model -- Intra-bone Bone Marrow Transplantation in Pig-to-Non-human Primates for the Induction of Tolerance Across Xenogeneic Barriers -- Studying Xenograft Rejection of Bioprosthetic Heart Valves -- Corneal Xenotransplantation: Anterior Lamellar Keratoplasty -- Rat Model of Intra-articular Chondrocyte Xenotransplantation -- Porcine Hepatocytes: Isolation and Liver Tissue Engineering for Xenotransplantation -- Pig-to-Macaque Islet Xenotransplantation -- Regulation of Clinical Xenotransplantation: A Reappraisal of the Legal, Ethical and Social Aspects Involved. .
520			_aThis second edition volume delivers updated and new chapters on xenotransplantation covering a variety of methods. Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in the last three decades. Expert researchers provide characterizations of xenogeneic interactions at the cellular and molecular levels, detailed descriptions of complex transplantation models, as well as attractive new tools for further advancement including 3D cell-culturing systems and genomic editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, Xenotransplantation: Methods and Protocol, Second Edition aids scientists in continuing to study xenotransplantation and its multiple aspects.
700	1		_aCosta, Cristina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071602546
776	0	8	_iPrinted edition: _z9781071602560
776	0	8	_iPrinted edition: _z9781071602577
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0255-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233020 _d233020