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008			161206s2017 xxu| s |||| 0|eng d
020			_a9781493965632
024	7		_a10.1007/978-1-4939-6563-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDrug Target miRNA _bMethods and Protocols _cedited by Marco F. Schmidt.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 319 páginas) _b94 ilustraciones, 62 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1517
505	0		_aMiRNA Targeting Drugs: The Next Blockbusters? -- Functional Analysis of miRNA using DIANA Tools Online Suite -- Non-Nucleotide Modification of Anti-miRNA Oligonucleotides -- Quantification of Oligonucleotide Association with miRNA-Argonaute Complexes In Vitro -- Determination of Anti-miR Association with miRNA/Argonaute Complexes In Vivo -- Competitive Argonaute-Based RNA Immunoprecipitation for Investigation of Transcriptomic Response to Anti-miR -- Assessing Anti-miR Pharmacology with miRNA Polysome Shift Assay -- Evaluating Synergistic Effects of miR-34a Mimics in Combination with Other Therapeutic Agents in Cultured Non-Small Cell Lung Cancer Cells -- Assessing the Off-Target Effects of miRNA Inhibitors on Innate Immune Toll-Like Receptors -- Design of Multimodal Small Molecules Targeting miRNA Biogenesis: Synthesis and In Vitro Evaluation -- Machine Learning Approaches towards Building Predictive Models for Small Molecule Modulators of miRNA and its Utility in Virtual Screening of Molecular Databases -- Identification of Small Molecule Modulators of MicroRNA by Library Screening -- Rapid Generation of miRNA Inhibitor Leads by Bio-Informatics and Efficient High-Throughput Screening Methods -- Peptide-Based Inhibition of miRNA-Guided Gene Silencing -- Small Molecules Targeting the miRNA Binding Domain of Argonaute 2: From Computer-Aided Molecular Design to RNA Immunoprecipitation -- Surface Plasmon Resonance: A Useful Strategy for the Identification of Small Molecule Argonaute 2 Protein Binders -- Antagonists of the miRNA-Argonaute 2 Protein Complex: Anti-miR-AGOs -- Elucidating Mechanisms of Molecular Recognition between Human Argonaute and miRNA using Computational Approaches -- Kinetic Analysis of Target RNA Binding and Slicing by Argonaute 2 Protein -- Site-Specific Fluorescent Labeling of Argonaute for FRET-Based Bio-Assays -- Single-Molecule Fluorescence Energy Transfer Assays for the Characterization of Reaction Pathways of miRNA-Argonaute Complex.
520			_aThis volume provides a concise and technical discussion of recently developed approaches to overcome challenges, such as pharmacodynamics and pharmacokinetics difficulties, in miRNA drug discovery. These strategies cover anti-sense agents targeting miRNA that are applied in advanced formulations or are chemically optimized to increase delivery; small molecule miRNA modulators to overcome anti-sense agents' limitations; general enhancers of miRNA maturation; and Argonaute 2 protein and its pharmacokinetic parameters. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Drug Target miRNA: Methods and Protocols is a valuable resource for anyone interested in the ever-evolving field of miRNA drug discovery. .
700	1		_aSchmidt, Marco F _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493965618
776	0	8	_iPrinted edition: _z9781493965625
776	0	8	_iPrinted edition: _z9781493982363
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6563-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233048 _d233048