000 04213nam a22003735i 4500
001 233051
003 ES-VaUE
005 20221220020435.0
007 cr nn 008mamaa
008 161121s2017 xxu| s |||| 0|eng d
020 _a9781493965847
024 7 _a10.1007/978-1-4939-6584-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aSmall Molecule Microarrays
_bMethods and Protocols
_cedited by Mahesh Uttamchandani, Shao Q. Yao.
250 _a2nd edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XI, 260 páginas)
_b82 ilustraciones, 60 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1518
505 0 _aThe Expanding World of Small Molecule Microarrays -- Novel Substrates for Microarrays -- Fabrication of Bio-function-preserved Saccharide Microarray Chips with Cyanuric Chloride as a Rotatable Linker -- Fabrication of Carbohydrate Microarrays by Boronate Formation -- Clickable Polymeric Coating for Glycan Microarrays -- A Versatile Microarray Immobilization Strategy Based on a Biorthogonal Reaction between Tetrazine and Trans-Cyclooctene -- Label-Free Sensing on Microarrays -- Optical Microscopy for Detecting Binding on Small Molecule Microarrays -- Array-on-array Strategy For Activity-based Enzyme Profiling -- Protein-Protein Interaction Inhibitors of BRCA1 Discovered Using Small Molecule Microarrays -- Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays -- Profiling Phosphopeptide-binding Domain Recognition Specificity using Peptide Microarrays -- Validation Procedure for Multiplex Antibiotic Immunoassays Using Flow-based Chemiluminescence Microarrays -- Large-scale Interaction Profiling of Protein Domains through Proteomic Peptide-PhageDisplay using Custom Peptidomes -- Synthetic Glycan Microarrays -- Screening Mammalian Cells on a Hydrogel- Functionalized Small Molecule Microarray.
520 _aSmall molecule microarrays (SMM) were introduced in 1999 and within just a short span of time, have established themselves as a vibrant next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols consolidates a significant collection of examples, which serve to guide researchers toward ways in which SMM technology may be effectively deployed for multiplexed screening, drug discovery and ligand identification. The volume is organized into three sections covering microarray fabrication, imaging modes, and discovery approaches. The chapters cover a range of small molecule library types, ranging from synthetic combinatorial libraries to peptide and carbohydrate libraries, with lessons and techniques that can be widely applied. Written in the highly successful Methods in Molecular Biology series, format, chapters include introductions of their respective topics, lists of the requisite materials and reagents, step-by-step and readily reproducible laboratory protocols as well as vital tips on troubleshooting and advice on avoiding known pitfalls. Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous insights into the successful applications of SMM, by experts in the field. Building on this collection of knowledge, the volume seeks to inspire a future generation of microarray practitioners to propel this significant technology to even greater heights .
700 1 _aUttamchandani, Mahesh
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aYao, Shao Q
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493965823
776 0 8 _iPrinted edition:
_z9781493965830
776 0 8 _iPrinted edition:
_z9781493982424
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6584-7
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2017
999 _c233051
_d233051