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008			161216s2017 xxu| s |||| 0|eng d
020			_a9781493964819
024	7		_a10.1007/978-1-4939-6481-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRNA Vaccines _bMethods and Protocols _cedited by Thomas Kramps, Knut Elbers.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 282 páginas) _b42 ilustraciones, 26 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1499
505	0		_aIntroduction to RNA Vaccines -- Self-replicating RNA -- Self-replicating RNA Vaccine Delivery to Dendritic Cells -- Plant Expression of Trans-encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons -- RNActive® Technology: Generation and Testing of Stable and Immunogenic mRNA Vaccines -- Nucleoside Modified mRNA Vaccines for Infectious Diseases -- Generation and Evaluation of Prophylactic mRNA Vaccines against Allergy -- Measuring the Adjuvant Activity of RNA Vaccines -- Generation of Immunostimulating 130 nm Protamine-RNA Nanoparticles -- Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins -- Adjuvant-Enhanced mRNA Vaccines -- Enhanced Delivery of DNA or RNA Vaccines by Electroporation -- The European Regulatory Environment of RNA-Based Vaccines -- Discovery and Subtyping of Neo-Epitope Specific T-cell Responses for Cancer Immunotherapy: Addressing the Mutanome -- Considerations for Producing mRNA Vaccines for Clinical Trials -- Nonclinical Safety Testing of RNA Vaccines -- Immunotherapy of Uveal Melanoma: Vaccination Against Cancer.
520			_aThis volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinical development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Vaccines: Methods and Protocols aims to increased collaboration on RNA vaccines between basic and applied scientists in academia, government, and industry to develop future solutions for today's challenges.
700	1		_aKramps, Thomas _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aElbers, Knut _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493964796
776	0	8	_iPrinted edition: _z9781493964802
776	0	8	_iPrinted edition: _z9781493982141
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6481-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233054 _d233054