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008			170429s2017 xxu| s |||| 0|eng d
020			_a9781493969340
024	7		_a10.1007/978-1-4939-6934-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aLysosomes _bMethods and Protocols _cedited by Karin Öllinger, Hanna Appelqvist.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XIII, 311 páginas) _b78 ilustraciones, 61 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1594
505	0		_aSILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS -- Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins -- Analyzing Lysosome Related Organelles By Electron Microscopy -- Microscopic Analysis of Lysosomal Membrane Permeabilization -- Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets -- Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues -- Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent Recovery -- Delivery of Cargo to Lysosomes using Gneosomes -- Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles -- Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded Cells -- Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry -- Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes -- Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and Substrates -- Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of Lysoplex -- Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L Forms -- Methods for Determination of Α-Glycosidase, Β-Glycosidase, and Α-Galactosidase Activities in Dried Blood Spot Samples -- Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi -- Lysosomal Biology in Cancer. .
520			_aThis volume provides laboratory protocols essential for studies on lysosomal biology. Chapters aim to guide researchers in their exploration of lysosomes, both under normal conditions and in pathological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lysosome: Methods and Protocols aims to provided protocols that will guide and inspire further research and generate new insights into this fascinating organelle.
700	1		_aÖllinger, Karin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aAppelqvist, Hanna _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493969326
776	0	8	_iPrinted edition: _z9781493969333
776	0	8	_iPrinted edition: _z9781493983438
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6934-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233055 _d233055