000			03442nam a22003615i 4500
001			233056
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592591886
024	7		_a10.1385/1592591884 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aApoptosis Techniques and Protocols _cedited by Andréa C. LeBlanc.
250			_a2nd edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XV, 250 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v37
505	0		_aMolecular Characterization of the Proapoptotic Protein Bax -- Assessing Cytochrome-c Release from Mitochondria -- Monitoring Caspases in Neuronal Cell Death -- HSV Amplicon Vectors in Neuronal Apoptosis Studies -- Microinjections to Study the Specific Role of Proapoptotic Proteins in Neurons -- Designing Microarray Experiments for Neurobiology -- Analysis of Gene Expression by Nylon Membrane cDNA Arrays -- Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs -- Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons -- Detection and Analysis of Synaptosis -- In Situ Detection of Apoptotic Neurons -- Apoptotic and Oxidative Indicators in Alzheimer's Disease.
520			_aThe study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocol includes tips on avoiding pitfalls, notes on the method's advantages and disadvantages, and a critical survey of the literature. Cutting-edge and highly practical, Apoptosis Techniques and Protocols, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death.
700	1		_aLeBlanc, Andréa C _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617373060
776	0	8	_iPrinted edition: _z9781588290120
776	0	8	_iPrinted edition: _z9781489938855
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591884 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c233056 _d233056