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008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592591787
024	7		_a10.1385/1592591787 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTransgenesis Techniques _bPrinciples and Protocols _cedited by Alan R. Clarke.
250			_a2nd edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (X, 351 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v180
505	0		_aTopical Reviews in Transgenesis -- Biomedical and Agricultural Applications of Animal Transgenesis -- Transgenesis in Invertebrate and Lower Vertebrate Species -- Gene Transfer in Drosophila -- Transgenesis in the Mouse: Oocyte Injection -- Oocyte Injection in the Mouse -- Alternative Routes to the Germline -- Adenoviral Infection -- Retroviral Infection -- Transgenesis in the Mouse: The ES Cell Route -- In Vitro Isolation of Murine Embryonic Stem Cells -- Production of Chimeras Derived from Murine Embryonic Stem Cells -- Gene-Targeting Strategies -- Cre/loxP Recombination System and Gene Targeting -- Cryopreservation of Mouse Lines -- Cryopreservation of Transgenic Mouse Lines -- Ovarian Tissue Transplantation and Cryopreservation -- Transgenesis in the Rat -- Transgenesis in the Rat -- Transgenesis in Domestic Species -- Generation of Transgenic Livestock by Pronuclear Injection -- Transgenic Sheep from Cultured Cells -- Characterization and Analysis of Transgenic Strains -- Analysis of Transgenic Mice.
520			_aThe past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes.
700	1		_aClarke, Alan R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489939012
776	0	8	_iPrinted edition: _z9781489939005
776	0	8	_iPrinted edition: _z9780896036963
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591787 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c233060 _d233060