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020 _a9781592595396
024 7 _a10.1385/0896033155
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
100 1 _aAltria, Kevin D.
_eautor
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
245 1 0 _aCapillary Electrophoresis Guidebook
_bPrinciples, Operation, and Applications
_cby Kevin D. Altria.
250 _a1st edition 1996
264 1 _aTotowa, NJ
_bHumana Press
_c1996
300 _a1 recurso en línea (IX, 349 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v52
505 0 _aGeneral Guidelines to the Operation of Capillary Electrophoresis Methods and Instrumentation -- Fundamentals of Capillary Electrophoresis Theory -- Standard Commercial Instrument Description -- Typical Operating Procedures -- Method Development/Optimization -- Quantitation Procedures -- Optimization of Precision in Quantitative Analysis -- Optimization of Sensitivity -- Method Validation -- Fraction Collection -- Troubleshooting -- Quick Guide to Running a Successful Separation -- Applications of Capillary Electrophoresis and Specific Technologies -- Micellar Electrokinetic Chromatography -- Capillary Gel Electrophoresis -- Chiral Separations by Capillary Electrophoresis -- Capillary Electrochromatography -- Application and Limits of Sample Stacking in Capillary Electrophoresis -- Analysis of Bases, Nucleosides, and (Oligo)nucleotides by Capillary Electrophoresis -- Application of Capillary Electrophoresis to Pharmaceutical Analysis -- Separation of Peptides and Protein Digests by Capillary Electrophoresis -- Additional Application Areas of Capillary Electrophoresis.
520 _aThis book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap­ idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro­ vided for individual analyses. The intention is to provide general guide­ lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth­ ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.
776 0 8 _iPrinted edition:
_z9781617370113
776 0 8 _iPrinted edition:
_z9780896033153
776 0 8 _iPrinted edition:
_z9781489940285
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033155
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_1996
999 _c233061
_d233061