000			03698nam a22003615i 4500
001			233062
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s1998 xxu| s |||| 0|eng d
020			_a9781592595723
024	7		_a10.1385/0896034879 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProtein Targeting Protocols _cedited by Roger A. Clegg.
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XII, 331 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v88
505	0		_aSurface Plasmon Resonance -- Microcalorimetry of Protein-Protein Interactions -- Partitioning of Proteins in Triton X-114 -- Co-Immunoprecipitation -- Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring -- Size-Exclusion Chromatography -- Overlay and Bead Assay -- Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins -- Molecular Genetic Approaches I -- Molecular Genetic Approaches II -- Molecular Genetic Approaches III -- Molecular Genetic Approaches IV -- Use of Synthetic Peptides in the Dissection of Protein-Targeting Interactions -- SH2 and SH3 Domains -- PH Domain of Serine-Threonine Protein Kinase B (RAC-PKB) -- Membrane Targeting via Protein N-Myristoylation -- Membrane Targeting via Protein Palmitoylation -- Agonist-Mediated Turnover of G-Protein ?-Subunit Palmitoyl Groups -- Membrane Targeting via Protein Prenylation -- Nuclear Protein Import in a Permeabilized Cell Assay -- Clathrin-Coated Vesicles and Targeting -- Analysis of the Sorting of Secretory Proteins to the Regulated Secretory Pathway.
520			_aIn Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored. Protein Targeting Protocols offers a valuable collection of both basic and state-of-the-art techniques suitable for use in all areas of molecular cell biology by researchers interested in defining and characterizing the wide variety of protein interactions underlying targeting mechanisms. Readers will find it serves well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it.
700	1		_aClegg, Roger A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489942999
776	0	8	_iPrinted edition: _z9780896034501
776	0	8	_iPrinted edition: _z9780896034877
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034879 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1998
999			_c233062 _d233062