000			04038nam a22003615i 4500
001			233066
003			ES-VaUE
005			20221220020436.0
007			cr nn 008mamaa
008			180705s2018 xxu| s |||| 0|eng d
020			_a9781493977864
024	7		_a10.1007/978-1-4939-7786-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPhotosynthesis _bMethods and Protocols _cedited by Sarah Covshoff.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 355 páginas) _b70 ilustraciones, 47 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1770
505	0		_aSurvey of Tools for Measuring In Vivo Photosynthesis -- Photosynthetic Gas Exchange in Land Plants at the Leaf Level -- Design and Use of a Digitally-Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants -- Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS) -- Light-Response Curves in Land Plants -- Chlorophyll Fluorescence on the Fast Timescale -- Sub-Saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield -- Chlorophyll Fluorescence Imaging -- Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry -- Using Carbon Stable Isotopes to Study C3 and C4 Photosynthesis: Models and Calculations.- Liquid-Phase Measurements of Photosynthetic Oxygen Evolution.- Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting -- Extraction of Rubisco to Determine Catalytic Constants -- Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts -- Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features.- Determining the Subcellular Localization of Fluorescently-Tagged Proteins Using Protoplasts Extracted from Transiently-Transformed Nicotiana benthamiana Leaves.- 3D Clearing and Molecular Labeling in Plant Tissues -- Evaluation of Lipids for the Study of Photosynthetic Membranes -- "Click" Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs -- Biophotovoltaics: Design and Study of Bio-Electrochemical Systems for Biotechnological Applications and Metabolic Investigation.
520			_aThis user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis.
700	1		_aCovshoff, Sarah _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493977857
776	0	8	_iPrinted edition: _z9781493977871
776	0	8	_iPrinted edition: _z9781493992850
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7786-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233066 _d233066