000			03609nam a22003615i 4500
001			233069
003			ES-VaUE
005			20221220020436.0
007			cr nn 008mamaa
008			180725s2018 xxu| s |||| 0|eng d
020			_a9781493986859
024	7		_a10.1007/978-1-4939-8685-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aVibrio Cholerae _bMethods and Protocols _cedited by Aleksandra E. Sikora.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (X, 180 páginas) _b27 ilustraciones, 19 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1839
505	0		_aLaboratory Culturing Techniques and Maintenance of Vibrio cholera -- Genotypic and Phenotypic Assays to Distinguish Vibrio cholera Biotype -- Preparation of Vibrio cholera Samples for RNA-seq Analysis -- Random Transposon Mutagenesis of Vibrio cholera -- Metabolomics of Vibrio cholera -- Natural Cotransformation and Multiplxed Genome Editing by Natural Transformation (MuGENT) of Vibrio cholera -- Vibrio cholera Chromatin Immunoprecipitation -- Fly Models of Vibrio cholera Infection and Colonization -- Danio rerio as a Native Host Model for Understanding Pathophysiology of Vibrio cholera -- Transposon Sequencing of Vibrio cholera in the Infant Rabbit Model of Cholera -- Isolation of Outer Membrane Vesicles Including their Quantitative and Qualitative Analyses -- Utilization of Vibrio cholera as a Model Organism to Screen natural Product Libraries for Identification of New Antibodies -- Infant Mouse Model of Vibrio cholera Infection and Colonization -- Methods for Assessments of Collagenolytic Activity of the Vibrio cholera Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin -- Proteomics of Vibrio cholerarea.
520			_aThis volume looks at the importance of V. cholerae as an infection agent, and explores the latest techniques used to study it. The chapters in this book cover topics such as the biology of V. cholerae; laboratory maintenance and culturing; various animals models developed to explain pathogenesis and factors for environmental survival; and holistic approaches that include proteomics, metabolomics, and high-throughput screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Vibrio cholerae: Methods and Protocols is a valuable resource for both novice microbiologists who want to focus their studies on V. cholerae, and experienced researchers and teachers who want to improve their knowledge and skills in this growing field.
700	1		_aSikora, Aleksandra E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493986842
776	0	8	_iPrinted edition: _z9781493986866
776	0	8	_iPrinted edition: _z9781493993659
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8685-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233069 _d233069