000			04500nam a22003615i 4500
001			233072
003			ES-VaUE
005			20221220020436.0
007			cr nn 008mamaa
008			180810s2018 xxu| s |||| 0|eng d
020			_a9781493985883
024	7		_a10.1007/978-1-4939-8588-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aADP-ribosylation and NAD+ Utilizing Enzymes _bMethods and Protocols _cedited by Paul Chang.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIV, 417 páginas) _b95 ilustraciones, 51 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1813
505	0		_aVitamin B3 in Health and Disease: Toward the Second Century of Discovery -- Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently-Tagged Macrodomains -- In Vitro Techniques for ADP-Ribosylated Substrate Identification -- Assessment of Intracellular Auto-Modification Levels of ARTD10 using Mono-ADP-Ribose-Specific Macrodomains 2 and 3 of Murine Artd8 -- Biochemical and Biophysical Assays of PAR-WWE Domain Interactions and Production of iso-ADPr for PAR Binding Analysis -- Assays for NAD-Dependent Reactions and NAD Metabolites -- Generating Protein-Linked and Protein-Free Mono-, Oligo-, and Poly(ADP-Ribose) In Vitro -- Methods to Study TCDD-Inducible Poly-ADP-Ribose Polymerase (TIPARP) Mono-ADP-Ribosyltransferase Activity -- Dictyostelium as a Model to Assess Site-Specific ADP-Ribosylation Events -- Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases -- Monitoring Expression and Enzyme Activity of Ecto-ARTCs -- ADP-Ribosyl-Acceptor Hydrolase Activities Catalyzed by the ARH Family of Proteins -- Mono-ADP-Ribosylhydrolase Assays -- Hydrolysis of ADP-Ribosylation by Macrodomains -- HPLC-Based Enzyme Assays for Sirtuins -- Small Molecule Screening Assay for Mono-ADP-Ribosyltransferases -- A Simple, Sensitive, and Generalizable Plate Assay for Screening PARP Inhibitors -- Non-Localized Searching of HCD Data for Fast and Sensitive Identification of ADP-Ribosylated Peptides -- Quantitative Determination of MAR Hydrolase Residue Specificity In Vitro by Tandem Mass Spectrometry -- Detection of ADP-Ribosylating Bacterial Toxins -- Preparation of Recombinant Alphaviruses for Functional Studies of ADP-Ribosylation -- Monitoring the Sensitivity of T Cell Populations towards NAD+ Released during Cell Preparation -- Identifying Target RNAs of PARPs -- ADPr-Peptide Synthesis -- Identifying Genomic Sites of ADP-Ribosylation Mediated by Specific Nuclear PARP Enzymes Using Click-ChIP -- Methods for Using a Genetically-Encoded Fluorescent Biosensor to Monitor Nuclear NAD+.
520			_aThis volume focuses on mono-ADP-ribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADP-ribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key protocols used to study topics such as in vitro techniques for ADP-ribosylation substrate identification; biochemical and biophysical assays of PAR-WWE domain interactions; monitoring expression and enzyme activity of ecto-ARTCs; HPLC-based enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, ADP-ribosylation and NAD+ Utilizing Enzymes: Methods and Protocols is a valuable resource for anyone interested in this developing and expanding field.
700	1		_aChang, Paul _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493985876
776	0	8	_iPrinted edition: _z9781493985890
776	0	8	_iPrinted edition: _z9781493993352
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8588-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233072 _d233072