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| 001 | 233073 | ||
| 003 | ES-VaUE | ||
| 005 | 20221220020436.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 180810s2018 xxu| s |||| 0|eng d | ||
| 020 | _a9781493987450 | ||
| 024 | 7 |
_a10.1007/978-1-4939-8745-0 _2doi |
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| 040 |
_aES-MaUEC _bspa _cES-MaUEC |
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| 245 | 1 | 0 |
_aProtein Gel Detection and Imaging _bMethods and Protocols _cedited by Biji T. Kurien, R. Hal Scofield. |
| 250 | _a1st edition 2018 | ||
| 264 | 1 |
_aNew York, NY _bSpringer International Publishing _c2018 |
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| 300 |
_a1 recurso en línea (XIII, 289 páginas) _b39 ilustraciones, 35 ilustraciones a color |
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| 336 |
_atexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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| 490 | 0 |
_aMethods in Molecular Biology _x1940-6029 _v1853 |
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| 505 | 0 | _aProtein Stains and Applications -- The Roles of Acetic Acid and Methanol during Fixing and Staining Proteins in a SDS Polyacrylamide Electrophoresis Gel -- Multicolored Prestained Standard Protein Marker Generation using a Variety of Remazol Dyes for Easy Visualization of Protein Bands during SDS-PAGE -- Coomassie-Brilliant Blue Staining of Polyacrylamide Gels -- A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate Polyacrylamide Gel using Coomassie Blue -- Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue -- Silver Staining Techniques of Polyacrylamide Gels -- Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry -- Detection of Phosphoproteins in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis using 8-Quinolinol Stain -- Microwave Assisted Protein Staining, De-Staining and In-Gel/In-Solution Digestion of Proteins -- Fluorescent Staining of Gels -- A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta during Western Blot Analysis -- TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE -- Detection of Glycoproteins in Polyacrylamide Gels using Pro-Q Emerald 300 Dye, A Fluorescent Periodate Schiff-Base Stain -- Curcumin/Turmeric as an Environment-Friendly Protein Gel Stain -- Detection of Multiple Enzymes in Fermentation Broth using Single PAGE Analysis -- Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis -- A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins -- Tem Minute stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct red 81 and Amido Black -- In-Gel Protein Phosphatase Assay using Fluorogenic Substrates -- Detection of Proteins in Polyacrylamide Gels via Pre-Labeling by Isatoic Anhydride -- Fluorescent Protein Visualization Immediately after Gel Electrophoresis using an In-Gel Trichloroethanol Photoreaction with Tryptophan -- Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel -- Zymographic Determination of Intrinsic Specific Activity of Oxidases in Presence of Interfering Proteins -- A Simple Method for Detecting Phosphorylation of Proteins by using ZN2+ -Phos-tag SDS-PAGE at Neutral pH -- Principle and Method of Silver Staining of Proteins Separated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis -- Heat/Pressure Treatment with Detergents Significantly Increases Curcumin Solubility and Stability: Its Use as an Environment-Friendly Protein Gel Stain -- Fungal Laccase Efficiently Destains Coomassie Brilliant Blue-R-250 Stained Polyacrylamide Gels -- Destaining Coomassie Brilliant Blue-Stained Sodium Dodecyl Sulfate Polyacrylamide Protein Gels using a Household Detergent -- Paper Adsorbents Remove Coomassie Blue from Gel Destain and used Gel Stain in an Environment-Friendly Manner -- Gel Drying Methods -- Stained Gels can be Stored for Several Months in Non-Sealed Polyethylene Bags -- Radiolabeling and Analysis of Labeled Proteins. | |
| 520 | _aThis volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field. | ||
| 700 | 1 |
_aKurien, Biji T _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 700 | 1 |
_aScofield, R. Hal _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 776 | 0 | 8 |
_iPrinted edition: _z9781493987443 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781493987467 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781493993819 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8745-0 _z(usuarios Universidad Europea de Valencia) |
| 942 |
_2lcc _cLE |
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| 988 | _aSpringer_Protocols_2018 | ||
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_c233073 _d233073 |
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