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008			180814s2018 xxu| s |||| 0|eng d
020			_a9781493986729
024	7		_a10.1007/978-1-4939-8672-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aLipases and Phospholipases _bMethods and Protocols _cedited by Georgina Sandoval.
250			_a2nd edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIV, 437 páginas) _b89 ilustraciones, 57 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1835
505	0		_aPart 1. Overview of Lipases, Phospholipases and Esterases -- Lipases: An Overview -- Carbohydrate Esterases: An Overview -- Phospholipases: An Overview -- Part 2. Activity Screening and Expression -- Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates -- A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities using α-Eleostearic Acid-Containing Substrates -- Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced Fluorescence Property of 8-Hydroxyquinoline -- Lipase and Phospholipase Activity Methods for Marine Organisms -- Plant Lipases: Functional Expression of CPLIP1 from Carica papaya -- Heterologous Expression and Functional Characterization of Sparidae Fish Digestive Phospholipase A2 -- Expression and Purification of Recombinant Vigna unguiculata Phospholipase D in Pichia pastoris for Structural Studies -- Part 3. Optimization of the Biocatalyst Production and Performances -- Recent Advances in Pichia pastoris as Host for Heterologous Expression Systems for Lipases (Review) -- Solid-State Fermentation as an Economic Production Method of Lipases and Esterases -- Synthetic Biology to Improve the Production of Lipases and Esterases (Review) -- Rational Design Strategy as a Novel Immobilization Methodology Applied to Lipases and Phospholipases -- Part 4. Applications of Lipases, Phospholipases and Esterases -- Lipase-Catalyzed Synthesis of Fatty Acid Esters of Trisaccharides -- Targeting Phospholipase D genetically and Pharmacologically for Studying Leukocyte Function -- Lipase-Catalyzed Synthesis of Structured Lipids -- Lipase-Catalyzed Acetylation and Esterification of Bile Acids -- Lipases in Green Chemistry: Deep Eutectic Solvents (DES) as New Green Solvents -- Chemoenzymatic Synthesis of Nitrogen Polymers with Biomedical Applications Catalyzed by Lipases -- Lipases and Esterases as Biocatalyst for Biodiesel Production (Review) -- Lipases, Phospholipases and Esterases Biosensors (Review).
520			_aThis volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Edition serves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.
700	1		_aSandoval, Georgina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493986712
776	0	8	_iPrinted edition: _z9781493986736
776	0	8	_iPrinted edition: _z9781493993611
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8672-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233074 _d233074