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007			cr nn 008mamaa
008			180822s2018 xxu| s |||| 0|eng d
020			_a9781493987542
024	7		_a10.1007/978-1-4939-8754-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProgrammed Necrosis _bMethods and Protocols _cedited by Adrian T. Ting.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 191 páginas) _b42 ilustraciones, 12 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1857
505	0		_aTools in the Art of Studying Necroptosis -- Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules -- Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis -- Distinguishing Necroptosis from Apoptosis -- Methods for Studying TNF-Mediated Necroptosis in Cultured Cells -- Analysis of Necroptosis in Bone Marrow-Derived Macrophages -- Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis -- Detection of MLKL Oligomerization during Programmed Necrosis -- Analysis of Cytokine- and Influenza A Virus-Driven RIPK3 Necrosome Formation -- Detection of RIPK1 in the FADD-Containing Death Inducing Signaling Complex (DISC) during Necroptosis -- Use of RIP1 Kinase Small Molecule Inhibitors in Studying Necroptosis -- Analyzing Necroptosis using a RIPK1 Kinase Inactive Mouse Model of TNF Shock -- Assessment of In Vivo Kidney Cell Death: Acute Kidney Injury -- Assessment of In Vivo Kidney Cell Death: Glomerular Injury -- Detection of Necroptosis by Phospho-RIPK3 Immunohistochemical Labeling -- Characterization of the TNFR1-SC using 'Modified Tandem Affinity Purification' in Conjunction with Liquid Chromatography-Mass Spectrometry (LC-MS) -- Monitoring RIPK1 Phosphorylation in the TNFR1 Signaling Complex -- Analysis of CYLD Proteolysis by Caspase-8 in Bone Marrow-Derived Macrophages.
520			_aThis volume explores the latest techniques and approaches used to study this emerging form of cell death. The chapters in this book cover topics such as distinguishing necroptosis from apoptosis; detecting MLKL oligomerization during programmed necrosis; analyzing the RIPK3 necrosome complex; using RIPK1 kinase small molecule inhibitors in studying necroptosis; monitoring RIPK1 phosphorylation in the TNFR1 signaling complex; and assessing in vivo kidney cell death in glomerular injury. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Programmed Necrosis: Methods and Protocols is a valuable resource for technicians, graduate students, postdoctoral fellows, and other scientists who are pursuing studies in this field.
700	1		_aTing, Adrian T _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493987535
776	0	8	_iPrinted edition: _z9781493987559
776	0	8	_iPrinted edition: _z9781493993840
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8754-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233076 _d233076