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008			180823s2018 xxu| s |||| 0|eng d
020			_a9781493987092
024	7		_a10.1007/978-1-4939-8709-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTertiary Lymphoid Structures _bMethods and Protocols _cedited by Marie-Caroline Dieu-Nosjean.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 289 páginas) _b38 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1845
505	0		_aTertiary Lymphoid Structures among the World of Non-Canonical Ectopic Lymphoid Organizations -- Cellular and Vascular Components of Tertiary Lymphoid Structures -- Meningeal Immunity, Drainage, and Tertiary Lymphoid Structure Formation -- Development of Tools for the Selective Visualization and Quantification of TLS-Immune Cells on Tissue Sections -- A Quantitative Pathology Approach to Analyze the Development of Human Cancer-Associated Tertiary Lymphoid Structures -- Multiplex Immunohistochemistry for Image Analysis of Tertiary Lymphoid Structures in Cancer -- Defining High Endothelial Venules and Tertiary Lymphoid Structures in Cancer -- Development of Methods for Selective Gene Expression Profiling in Tertiary Lymphoid Structure using Laser Capture Microdissection -- Quantifying Tertiary Lymphoid Structure-Associated Genes in Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues -- Generation of Recombinant Monoclonal Antibodies from Single B Cells Isolated from Synovial Tissue of Rheumatoid Arthritis Patients -- Designed Methods for the Sorting of Tertiary Lymphoid Structure-Immune Cell Populations -- Identification of Tertiary Lymphoid Structure-Associated Follicular Helper T Cells in Human Tumors and Tissues -- Exploring the Role of Tertiary Lymphoid Structures using a Mouse Model of Bacteria-Infected Lungs -- Identification and Characterization of Tertiary Lymphoid Structures in Murine Melanoma -- Investigating Tumor-Associated Tertiary Lymphoid Structures in Murine Lung Adenocarcinoma -- Targeting Tertiary Lymphoid structures for Tumor Immunotherapy.
520			_aThis volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations. Pre-clinical models are also discussed in detail to show how TLS structure, development, and maintenance can be targeted and studied in vivo. The chapters in this book cover topics such as humans and mice; strategies to quantify TLS in order to use it in stained tissue sections; classifying a gene signature form fixed and paraffin-embedded tissues; and development of murine inflammatory models to help look at TLS in the context of infection or malignancy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Tertiary Lymphoid Structures: Methods and Protocols is a valuable resource that increases the reader's knowledge on immune functions and how they will pave the way to future therapeutic applications.
700	1		_aDieu-Nosjean, Marie-Caroline _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493987085
776	0	8	_iPrinted edition: _z9781493987108
776	0	8	_iPrinted edition: _z9781493993727
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8709-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233077 _d233077