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001			233091
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007			cr nn 008mamaa
008			191227s2020 xxu| s |||| 0|eng d
020			_a9781071602119
024	7		_a10.1007/978-1-0716-0211-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMERS Coronavirus _bMethods and Protocols _cedited by Rahul Vijay.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 224 páginas) _b40 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2099
505	0		_aStudying Evolutionary Adaptation of MERS-CoV -- Evaluating MERS-CoV Entry Pathways -- Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing -- Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein -- Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV -- Deducing the Crystal Structure of MERS-CoV Helicase -- Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans -- Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR -- Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay -- Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera -- Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA -- Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced Acute Respiratory Distress Syndrome -- Development of a Mouse Adapted MERS Coronavirus -- Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection -- Evaluation of Activation and Inflammatory Activity of Myeloid Cells DuringPathogenic Human Coronavirus Infection -- Histopathologic Evaluation and Scoring of Viral Lung Infection. .
520			_aThis volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus and anti-viral factors, and mouse models for MERS -coronavirus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MERS Coronavirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aVijay, Rahul _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071602102
776	0	8	_iPrinted edition: _z9781071602126
776	0	8	_iPrinted edition: _z9781071602133
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0211-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233091 _d233091