000			03957nam a22003615i 4500
001			233093
003			ES-VaUE
005			20221220020438.0
007			cr nn 008mamaa
008			200120s2020 xxu| s |||| 0|eng d
020			_a9781071603017
024	7		_a10.1007/978-1-0716-0301-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aStem Cell Transcriptional Networks _bMethods and Protocols _cedited by Benjamin L. Kidder.
250			_a2nd edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 308 páginas) _b75 ilustraciones, 67 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2117
505	0		_aEpitope Tagging ChIP-seq of DNA Binding Proteins using CETCh-seq -- User-friendly and Interactive Analysis of ChIP-seq data using EaSeq -- Evaluation of 3D Chromatin Interactions using Hi-C -- MSTD for Detecting Topological Domains from 3D Genomic Maps -- Creating 2D Occupancy Plots using plot2DO -- Detection of Epigenetic Field Defects Using a Weighted Epigenetic Distance-based Method -- Profiling Cell Type Abundance and Expression in Bulk Tissues with CIBERSORTx -- Visualization of Single Cell RNA-Seq Data using t-SNE in R -- Use of SuperCT for Enhanced Characterization of Single-cell Transcriptomic Profiles -- Interactive Alternative Splicing Analysis of Human Stem Cells using psichomics -- Gene Ontology Semantic Similarity Analysis using GOSemSim -- Derivation of Maternogenic Epiblast Stem Cells from Haploid Embryos -- Derivation of LIF-independent Embryonic Stem Cells using Inducible OCT4 Expression -- Simultaneous Derivation of Embryonic and Trophoblast Stem Cells from Mouse Blastocysts -- Reprogramming Fibroblasts to Neural Stem Cells by Overexpression of the Transcription factor Ptf1a -- Reprogramming of Fibroblasts to Neural Stem Cells by a Chemical Cocktail -- Efficient RNA-based Reprogramming of Disease-associated Primary Human Fibroblasts into Induced Pluripotent Stem Cells -- Direct Reprogramming Of Mouse Embryonic Fibroblasts to Induced Trophoblast Stem (iTS) Cells -- Characterization of Arsenic-induced Cancer Stem-like Cells. .
520			_aThis second edition provides techniques used to study of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. Chapters detail next-generation sequencing technologies, data analysis, protocols on analysis and visualization of single-cell RNA-Seq data, analysis of 3D chromatin architecture, interpretation of large-scale interaction networks, transcriptional networks in embryonic and adult stem cells, derivation of stem cells, and transcriptional programs that promote reprogramming, transdifferentiation, and cancer formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Stem Cell Transcriptional Network: Methods and Protocols, Second Edition aims to provide a key resource for biologists seeking to interrogate these vital networks.
700	1		_aKidder, Benjamin L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071603000
776	0	8	_iPrinted edition: _z9781071603024
776	0	8	_iPrinted edition: _z9781071603031
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0301-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233093 _d233093