000			04307nam a22003615i 4500
001			233097
003			ES-VaUE
005			20221220020438.0
007			cr nn 008mamaa
008			200306s2020 xxu| s |||| 0|eng d
020			_a9781071604342
024	7		_a10.1007/978-1-0716-0434-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aExpressed Protein Ligation _bMethods and Protocols _cedited by Miquel Vila-Perelló.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (X, 362 páginas) _b95 ilustraciones, 67 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2133
505	0		_a Methods and Applications of Expressed Protein Ligation -- Protein Splicing: From the Foundations to the Development of Biotechnological Applications -- Identification, Characterization, and Optimization of Split Inteins -- Methods to Study the Structure and Catalytic Activity of cis-splicing Inteins -- Expressed Protein Ligation: General Experimental Protocols -- Peptide Hydrazides as Thioester Equivalents for the Chemical Synthesis of Proteins -- Native Chemical Ligation Via N-Acylurea Thioester Surrogates Obtained by Fmoc Solid Phase Peptide Synthesis.-Fused Split Inteins: Tools for Introducing Multiple Protein Modifications -- Streamlined Expressed Protein Ligation: Site-specific Antibody-drug Conjugate -- In vivo Histone Labeling Using Ultrafast trans-Splicing Inteins -- Site-Directed Modification of Yeast-Produced Proteins Using Expressed Protein Ligation -- Site-specific Phosphorylation of PDZ Domains -- Semisynthesis and Reconstitution of Nucleosomes Carrying Asymmetric Histone Modifications -- Facile Semisynthesis of Ubiquitylated Peptides with the Ligation Auxiliary 2-Aminooxyethanethiol -- Investigating the Effects of O-GlcNAc Modifications in Parkinson's Disease using Semi-synthetic α-synuclein -- Recombinant Expression of Cyclotides Using Expressed Protein Ligation -- Highly Precise Protein Semisynthesis through Ligation-Desulfurization Chemistry in Combination with Phenacyl Protection of Native Cysteines. .
520			_aThis book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.
700	1		_aVila-Perelló, Miquel _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071604335
776	0	8	_iPrinted edition: _z9781071604359
776	0	8	_iPrinted edition: _z9781071604366
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0434-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233097 _d233097