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001			233104
003			ES-VaUE
005			20221220020438.0
007			cr nn 008mamaa
008			200702s2020 xxu| s |||| 0|eng d
020			_a9781071606216
024	7		_a10.1007/978-1-0716-0621-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Plant Cell Wall _bMethods and Protocols _cedited by Zoë A. Popper.
250			_a2nd edition 2020
264		1	_aNew York, NY _bSpringer International Publishing _c2020
300			_a1 recurso en línea (XVII, 544 páginas) _b141 ilustraciones, 102 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2149
505	0		_aHigh-Voltage Paper Electrophoresis (HVPE) -- Carbohydrate Gel Electrophoresis -- Capillary Electrophoresis with Detection by Laser-Induced Fluorescence -- Theory and Practice in Measuring In Vitro Extensibility of Growing Plant Cell Walls -- Formation of Cellulose-Based Composites with Hemicelluloses and Pectins using Komagataeibacter Fermentation -- Plant Tissue Cultures -- Protoplast Isolation and Manipulation in the Unicellular Model Plant, Penium margaritaceum -- Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens -- Expression of Cell-Wall Modifying Enzymes in Aspen for Improved Lignocellulose Processing -- Activity and Action of Cell-Wall Transglycanases -- Screening for Cellulolytic Plant Enzymes using Colorimetric and Fluorescence Methods -- Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls -- High-Resolution Imaging of Cellulose Organization in Cell Walls by Field Emission Scanning Electron Microscopy -- Analyzing Plant Cell Wall Ultrastructure by Scanning Near-Field Optical Microscopy (SNOM) -- Raman Imaging of Plant Cell Walls -- Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy -- Rapid Assessment of Cell Wall Polymer Distribution and Surface Topology of Arabidopsis Seedlings -- Analysis of Plant Cell Walls using High-Throughput Profiling Techniques with Multivariate Methods -- Vibratome Sectioning of Plant Materials for (Immuno)Cytochemical Staining -- Monoclonal Antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Cell Walls from Plants and Marine Algae -- Electron Tomography and Immunogold Labeling as Tools to Analyze de novo Assembly of Plant Cell Walls -- Contributions to Arabinogalactan Proteins Analysis -- In Situ/Subcellular Localization of Arabinogalactan Protein Expression by Fluorescent In Situ Hybridization, FISH -- Localization, Extraction, and Quantification of Plant and Algal Arabinogalactan Proteins -- Plant Cell Wall Proteomes: Bioinformatics and Cell Biology Tools to Assess the Bona Fide Cell Wall Localization of Proteins -- Bioinformatic Identification of Plant Hydroxyproline-Rich Glycoproteins -- Bioinformatics Analysis of Plant Cell Wall Evolution -- Automated Glycan Assembly of Plant Cell Wall Oligosaccharides -- Computerized Molecular Modelling of Carbohydrates.
520			_aThis second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and structure, plant tissue culture protoplast isolation, genetic manipulation, investigation of enzyme activities, and in situ localization of wall components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Plant Cell Wall: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists interested in learning more about this field. .
700	1		_aPopper, Zoë A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071606193
776	0	8	_iPrinted edition: _z9781071606209
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0621-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233104 _d233104