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020 _a9781071606605
024 7 _a10.1007/978-1-0716-0660-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Cold Acclimation
_bMethods and Protocols
_cedited by Dirk K. Hincha, Ellen Zuther.
250 _a2nd edition 2020
264 1 _aNew York, NY
_bSpringer International Publising
_c2020
300 _a1 recurso en línea (XII, 336 páginas)
_b92 ilustraciones, 64 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2156
505 0 _aIntroduction: Plant Cold Acclimation and Winter Survival -- Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays -- Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements -- Infrared Thermal Analysis of Plant Freezing Processes -- Conducting Field Trials for Frost Tolerance Breeding in Cereals -- A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes -- Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation -- Identification of Arabidopsis Mutants with Altered Freezing Tolerance -- Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues -- Using Pixel-Based Microscope Images to Generate 3-D Reconstructions of Frozen and Thawed Plant Tissue -- Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography -- Proteomic Approaches to Identify Proteins Responsive to Cold Stress -- Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins -- A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition -- Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry -- Determining the ROS and the Antioxidant Status of Leaves during Cold Acclimation -- Analysis of Changes in Plant Cell Wall Composition and Structure during Cold Acclimation -- Subcellular Compartmentation of Metabolites Involved in Cold Acclimation -- Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime -- Characterization of Ice Binding Proteins from Sea-Ice Microalgae -- Isolation and Characterization of Ice-Binding Proteins from Higher Plants.
520 _aThis detailed new edition combines a wide selection of experimental methods for plant cold acclimation, deacclimation, and cold memory, ranging from the whole plant level of ecology and breeding to molecular profiling and the detailed analysis of specific proteins, with many levels of investigated complexity in between. The collection updates methodology from the first edition while adding chapters on numerous key advances in the field. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Cold Acclimation: Methods and Protocols, Second Edition seeks to aid researchers, both new and experienced, in entering this exciting field of research or broadening the scope of their investigations.
700 1 _aHincha, Dirk K
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aZuther, Ellen
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071606599
776 0 8 _iPrinted edition:
_z9781071606612
776 0 8 _iPrinted edition:
_z9781071606629
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0660-5
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2020
999 _c233105
_d233105