000			03444nam a22003735i 4500
001			233107
003			ES-VaUE
005			20221220020438.0
007			cr nn 008mamaa
008			200630s2020 xxu| s |||| 0|eng d
020			_a9781071607046
024	7		_a10.1007/978-1-0716-0704-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aKupffer Cells _bMethods and Protocols _cedited by Myriam Aouadi, Valerio Azzimato.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (X, 160 páginas) _b34 ilustraciones, 24 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2164
505	0		_aKupffer Cell and Hepatocytes Isolation by Gradient Centrifugation from a Single Mouse Liver -- Kupffer Cell Isolation from Human Biopsies -- Liver Macrophage Isolation by Flow Cytometry Sorting -- Kupffer Cell Protein Isolation and Detection by Western Blot -- Kupffer Cell mRNA Sequencing -- Kupffer Cell and Monocyte-derived Macrophage Identification by Immunofluorescence on FFPE Mouse Liver Sections -- Sample Preparation of Optically Cleared Liver Tissue to Identify Liver Macrophages using 3D Microscopy -- Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer cells in mouse liver -- Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model -- Kupffer Cell Characterization by Mass Cytometry -- Enzymatic Quantification of Liver Lipids after Folch Extraction -- In vivo Investigation of High Fat Diet Induced Hepatic Lipid Dysfunctions -- An Obesogenic Dietary Mouse Model of Nonalcoholic Steatohepatitis -- Generating Hepatocellular Carcinoma in Mice By Expressing N-RasG12V Accompanied with Trp53 Silence Using A Transposon-based System Delivered via Hydrodynamic Ail Vein Injection -- Mouse Model of Alcoholic Steatohepatitis. .
520			_aThis volume provides a complete overview of kupffer cell biology, from isolation to phenotyping. Chapters guide readers through methods and protocols on phenotyping, including RNA sequencing, Mass cytometry and 3D microscopy, as well as gene manipulation in vivo, and disease models from fatty liver disease to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Kupffer Cells: Methods and Protocols  aims to ensure successful investigation of this growing field. .
700	1		_aAouadi, Myriam _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aAzzimato, Valerio _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071607039
776	0	8	_iPrinted edition: _z9781071607053
776	0	8	_iPrinted edition: _z9781071607060
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0704-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233107 _d233107