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008			200707s2020 xxu| s |||| 0|eng d
020			_a9781071604595
024	7		_a10.1007/978-1-0716-0459-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aLeptospira spp. _bMethods and Protocols _cedited by Nobuo Koizumi, Mathieu Picardeau.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (X, 291 páginas) _b66 ilustraciones, 56 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2134
505	0		_aIsolation and Culture of Leptospira from Clinical and Environmental Samples -- Core Genome Multi-Locus Sequence Typing Analyses of Leptospira spp. using the Bacterial Isolate Genome Sequence Database -- Use of MALDI-ToF Mass Spectrometry for Identification of Leptospira -- Cell Enumeration of Leptospira by Flow Cytometry -- The Single-Step Method of RNA Purification Applied to Leptospira -- Purification of LPS from Leptospira -- Isolation, Purification, and Characterization of Leptophages -- Creating a Library of Random Transposon Mutants in Leptospira -- Transposon Sequencing in Leptospira interrogans -- Specific Gene Silencing in Leptospira biflexa by RNA-Guided Catalytically inactive Cas9 (dCas9) -- Leptospira spp. Toolbox for Chemotaxis Assay -- In Situ Structural Analysis of Leptospira spp. by Electron Cryotomography -- Measurement of the Cell-Body Rotation of Leptospira -- In Vivo Imaging of Bioluminescent Leptospires -- Cell Monolayer Translocation Assay -- Cell Adhesion Assay to Study Leptospiral Proteins: An Approach to Investigate Host-Pathogen Interaction -- Complement Resistance Assays -- Evaluation of Intracellular Trafficking in Macrophages -- Biofilm Formation and Quantification using the 96-Microtiter Plate -- Survival Tests for Leptospira spp. -- Cultivation of Leptospira interrogans within Rat Peritoneal Dialysis Membrane Chambers -- Use of Golden Syrian Hamster as an Animal Model to Study Leptospirosis-Associated Immune Responses -- Evaluation of Vaccine Candidates against Leptospirosis using Golden Syrian Hamsters -- Leptospira and Leptospirosis -- Laboratory Diagnosis of Leptospirosis.
520			_aThis volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms. .
700	1		_aKoizumi, Nobuo _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPicardeau, Mathieu _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071604588
776	0	8	_iPrinted edition: _z9781071604601
776	0	8	_iPrinted edition: _z9781071604618
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0459-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233110 _d233110