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008			200828s2021 xxu| s |||| 0|eng d
020			_a9781071606681
024	7		_a10.1007/978-1-0716-0668-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCardiac Regeneration _bMethods and Protocols _cedited by Kenneth D. Poss, Bernhard Kühn.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIII, 346 páginas) _b83 ilustraciones, 69 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2158
505	0		_aMyocardial Infarction Techniques in Adult Mice -- Apical Resection and Cryoinjury in Neonatal Mouse Heart -- Left-Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice -- Myocardial Infarction in Pigs -- Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish -- Cardiac Resection Injury in Zebrafish -- A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish -- Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration -- Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes -- Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes -- Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells -- In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells -- Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs -- Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells -- Isolation and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts -- Ex Vivo Techniques to Study Heart Regeneration in Zebrafish -- Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters -- In Vivo Clonal Analysis of Cardiomyocytes -- High Fidelity Quantification of Cell Cycle Activity with Multi-Isotope Imaging Mass Spectrometry -- AAV Gene Transfer to the Heart -- In Vitro Synthesis of Modified RNA for Cardiac Gene Therapy -- Generation and Manipulation of Exosomes -- Epigenetic Assays in Purified Cardiomyocyte Nuclei -- Genetic Lineage Tracing of Non-Cardiomyocytes in Mice -- Experimental Hypoxia as a Model for Cardiac Regeneration in Mice.
520			_aThis detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models and methods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them. The chapter "Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
700	1		_aPoss, Kenneth D _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKühn, Bernhard _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071606674
776	0	8	_iPrinted edition: _z9781071606698
776	0	8	_iPrinted edition: _z9781071606704
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0668-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233113 _d233113