000			03380nam a22003735i 4500
001			233115
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005			20221220020439.0
007			cr nn 008mamaa
008			201029s2021 xxu| s |||| 0|eng d
020			_a9781071608180
024	7		_a10.1007/978-1-0716-0818-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPatch Clamp Electrophysiology _bMethods and Protocols _cedited by Mark Dallas, Damian Bell.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (X, 333 páginas) _b93 ilustraciones, 76 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2188
505	0		_aAn Introduction to Patch Clamp Recording -- Patch Clamp Technology in the 21st Century -- Heterologous Expression of Ion Channels in Mammalian Cell Lines -- Electrophysiology on Channel Forming Proteins In Artificial Lipid Bilayers: Next Generation Instrumentation For Multiple Recordings In Parallel -- Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology -- Multi-electrode Arrays -- Utilising Automated Electrophysiological Platforms in Epilepsy Research -- Dynamic Clamp on a Windows PC -- Combining Whole-cell Patch-clamp Techniques with Single-cell RNA Sequencing -- Bioluminescence Methodology for Ion Channel Studies -- Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons -- Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method -- In Vivo Patch Clamp Studies -- In Vivo Optogenetics with Stimulus Calibration -- Single or Double Patch Clamp Recordings in the Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics -- Optogenetics and Optical Tools in Automated Patch Clamping. .
520			_aThis volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be areference guide for current and future ion channel physiologists. .
700	1		_aDallas, Mark _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBell, Damian _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071608173
776	0	8	_iPrinted edition: _z9781071608197
776	0	8	_iPrinted edition: _z9781071608203
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0818-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233115 _d233115