000			04424nam a22003735i 4500
001			233116
003			ES-VaUE
005			20221220020439.0
007			cr nn 008mamaa
008			201030s2021 xxu| s |||| 0|eng d
020			_a9781071608920
024	7		_a10.1007/978-1-0716-0892-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aStructural Genomics _bGeneral Applications _cedited by Yu Wai Chen, Chin-Pang Bennu Yiu.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIV, 364 páginas) _b80 ilustraciones, 64 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2199
505	0		_aHigh-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis -- Rational Design and Construction of Active-Site Labeled Enzymes -- Screening and Production of Recombinant Human Proteins: Ligation Independent Cloning -- Screening and Production of Recombinant Human Proteins: Protein Production in E. coli -- Screening and Production of Recombinant Human Proteins: Protein Production in Insect Cells -- Expression Screening of Human Integral Membrane Proteins Using BacMam -- High-Throughput Expression Screening in Mammalian Suspension Cells -- In Vitro Production of Perdeuterated Proteins in H2O for Biomolecular NMR Studies -- Minimizing Heterogeneity of Protein Samples for Metal Transporter Proteins Using SAXS and Metal Radioisotopes -- Hydrogen/Deuterium Exchange-Mass Spectrometry for Probing Changes in Conformation and Dynamics of Proteins -- BeStSel: From Secondary Structure Analysis to Protein Fold Prediction by Circular Dichroism Spectroscopy -- Navigating the Global Protein-Protein Interaction Landscape Using iRefWeb -- State-of-the-Art Data Management: Improving the Reproducibility, Consistency, and Traceability of Structural Biology and In Vitro Biochemical Experiments -- Protein Structure Modeling with MODELLER -- Parameterization of a Dioxygen Binding Metal Site Using the MCPB.py Program -- Parameterization of Large Ligands for Gromacs Molecular Dynamics Simulation with LigParGen -- Simulation of Proteins Modified with a Fluorescent Label -- Protocol for Simulations of PEGylated Proteins with Martini 3 -- Molecular Data Visualization on Mobile Devices: A Quick Starter's Guide -- Molecular Data Visualization with Augmented Reality (AR) on Mobile Devices.
520			_aThis updated edition emphasizes the benefits of structural genomics to the wider structural research community with a diverse range of applicable methods. The book opens with an extensive section on protein production, including new methodologies for membrane and metal-binding proteins, as well as high-throughput protein production and screening. It continues with sections on structural analyses and data management, as well as modeling, simulation, and visualization. Overall, the spectrum of topics reflects the trend towards tackling more diverse challenges of studying macromolecular machineries and complexes. Written for the highly successful Methods in Molecular Biology series, chapters include the type of detail and key implementation advice to ensure successful results. Authoritative and up-to-date, Structural Genomics: General Applications, Second Edition serves as a vital guide to structural biologists in small to medium-sized laboratories seeking to better utilize this powerful area of study. Chapter 11 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. For further details see license information in the chapter.
700	1		_aChen, Yu Wai _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aYiu, Chin-Pang Bennu _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071608913
776	0	8	_iPrinted edition: _z9781071608937
776	0	8	_iPrinted edition: _z9781071608944
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0892-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233116 _d233116