000			03929nam a22003735i 4500
001			233117
003			ES-VaUE
005			20221220020439.0
007			cr nn 008mamaa
008			201111s2021 xxu| s |||| 0|eng d
020			_a9781071608807
024	7		_a10.1007/978-1-0716-0880-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aArabidopsis Protocols _cedited by Jose J. Sanchez-Serrano, Julio Salinas.
250			_a4th edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 465 páginas) _b16 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2200
505	0		_aHandling Arabidopsis and other Brassicaceae: Growth, Preservation of Seeds, Transformation and Genetic Crosses -- Bioinformatic Tools in Arabidopsis Research -- Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene -- Non-sterile Grafting Methods for Arabidopsis -- CRISPR/Cas9-based Genome Editing Toolbox for Arabidopsis thaliana -- Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration -- Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts -- Photosynthetic and Heterotrophic Cell Suspension Cultures -- Genome-wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals -- Visualising and Measuring Single Locus Dynamics in Arabidopsis thaliana -- Identification and Quantification of Small RNAs -- Flow Cytometry and Sorting in Arabidopsis -- Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development -- Using Genetically Encoded Fluorescent Biosensors for Quantitative in vivo Imaging -- Using Tripartite Split-sfGFP for the Study of Membrane Protein-Protein Interactions -- High-pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-labeling -- Atomic Force Microscopy to Study Cell Wall Mechanics in Plants -- Identification and Characterization of Reproductive Mutations in Arabidopsis -- Metabolomic Analysis of Natural Variation in Arabidopsis -- Untargeted Metabolomics of Arabidopsis Stomatal Immunity -- Biotin-based Proximity Labeling Of Protein Complexes in Planta -- Phosphoproteomic Analysis of Plant Membranes -- RNA-binding Protein Immunoprecipitation and High Throughput Sequencing. .
520			_aThis fourth edition compiles the most recent methodologies developed to exploit the Arabidopsis genome. Chapters detail access to public resources, to genetic, cell biology, biochemical and physiological techniques, and sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Arabidopsis Protocols, Fourth Edition aims to ensure successful results in the further study of this vital field.
700	1		_aSanchez-Serrano, Jose J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSalinas, Julio _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071608791
776	0	8	_iPrinted edition: _z9781071608814
776	0	8	_iPrinted edition: _z9781071608821
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0880-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233117 _d233117