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008			210302s2021 xxu| s |||| 0|eng d
020			_a9781071612743
024	7		_a10.1007/978-1-0716-1274-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBifidobacteria _bMethods and Protocols _cedited by Douwe van Sinderen, Marco Ventura.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (X, 239 páginas) _b26 ilustraciones, 18 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2278
505	0		_aMethods for the Isolation and Recovery of Bifidobacteria -- Bifidobacterium Transformation -- Isolation of Chromosomal and Plasmid DNA from Bifidobacteria -- Assembly, Annotation, and Comparative Analysis of Bifidobacterial Genomes -- Site-directed Mutagenesis of Bifidobacterium Strains -- Protocol to Select Bifidobacteria from Fecal and Environmental Samples -- Phageome Analysis of Bifidobacteria-Rich Samples -- Measuring Conjugated Linoleic Acid (CLA) Production by Bifidobacteria -- Detection, Isolation, and Purification of Bifidobacterial Exopolysaccharides -- Determination of Bifidobacterial Carbohydrate Utilisation Abilities and Associated Metabolic End-Products -- Model for Murine Gut Colonisation by Bifidobacteria -- Identification of Bifidobacteria by the Phosphoketolase Assay -- Determination of Bile Salt Hydrolase Activity in Bifidobacteria -- A Resource for Cloning and Expression Vectors Designed for Bifidobacteria: Overview of Available Tools and Biotechnological Applications -- Metagenomic Analyses of Bifidobacterial Communities -- Resistance of Bifidobacteria toward Antibiotics -- In vitro Assessment of Prebiotic Activity -- Bifidobacterium Genome Assembly and Methylome Analysis using Pacbio SMRT Sequencing. .
520			_aThis volume provides current protocols that can be used in various experimental settings involving bifidobacteria. Chapters guide readers through experimental protocols on procedures to isolate and cultivate bifidobacteria, taxonomic identification of bifidobacterial isolates, sequencing and annotate genomes, physiologically characterize bifidobacteria, and methods on the genetic manipulation of bifidobacterial strains. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bifidobacteria: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
700	1		_avan Sinderen, Douwe _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aVentura, Marco _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071612736
776	0	8	_iPrinted edition: _z9781071612750
776	0	8	_iPrinted edition: _z9781071612767
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1274-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233119 _d233119