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001			233121
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008			210303s2021 xxu| s |||| 0|eng d
020			_a9781071613702
024	7		_a10.1007/978-1-0716-1370-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Photomorphogenesis _bMethods and Protocols _cedited by Ruohe Yin, Ling Li, Kaijing Zuo.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 176 páginas) _b44 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2297
505	0		_aSetting up an Arabidopsis LED Culture Module that Simulates Plant Neighbor Proximity -- Photobody Detection Using Immunofluorescence and Super-Resolution Imaging in Arabidopsis -- Analysis of Shade-Induced Hypocotyl Elongation in Arabidopsis -- Isolation of UVR8 Protein Complexes -- Phenotypic Study of Photo-Morphogenesis in Arabidopsis Seedlings -- Experimental Procedures for Studying Skotomorphogenesis in Arabidopsis thaliana -- Global Identification for Targets of Circadian Transcription Factors in Arabidopsis and Rice Using Chromatin Immunoprecipitation Followed by Sequencing (ChIP-seq) -- Co-Immunoprecipitation Assays to Detect In Vivo Association of Phytochromes with Their Interacting Partners -- Detection of UVR8 Homodimers and Monomers by Immunoblotting Analysis in Tomato -- Characterization of Seedling Greening Process in Plant Photomorphogenesis -- Protoplast System for Studying Blue-Light-Dependent Formation of Cryptochrome Photobody -- Uncover the Nuclear Proteomic Landscape with Enriched Nuclei Followed by Label-Free Quantitative Mass Spectrometry -- Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii -- Co-Immunoprecipitation Assay for Blue Light-Dependent Protein Interactions in Plants -- Detecting Blue Light-Dependent Protein-Protein Interactions by LexA-Based Yeast Two-Hybrid Assay -- Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System -- Semi-In-Vivo Pull Down Assay for Blue Light-Dependent Protein Interactions -- Tobacco System for Studying Protein Colocalization and Interactions.
520			_aThis book provides detailed protocols for research in plant photomorphogenesis. The collection includes a broad range of topics including assays for shade avoidance responses, assays for light-dependent protein-protein interactions, photobody detection with immunofluorescence and the super-resolution imaging method, protein complex isolation from plants, detection of homodimer and monomer of photoreceptor UVR8 with immunoblotting analysis, assays for seedling greening, procedures for studying skotomorphogenesis, phenotypic study of photomorphogenesis at the seedling stage, expression of Cryptochrome in insect cells, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Photomorphogenesis: Methods and Protocols serves as an ideal guide for researchers and students who are new to the field, as well as a stepping stone for experienced researchers to further their skills in this fast-developing field.
700	1		_aYin, Ruohe _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLi, Ling _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aZuo, Kaijing _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071613696
776	0	8	_iPrinted edition: _z9781071613719
776	0	8	_iPrinted edition: _z9781071613726
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1370-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233121 _d233121