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020 _a9781071611500
024 7 _a10.1007/978-1-0716-1150-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aRibosome Profiling
_bMethods and Protocols
_cedited by Vyacheslav M. Labunskyy.
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XI, 348 páginas)
_b72 ilustraciones, 53 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2252
505 0 _aMonitoring Bacterial Translation Rates Genome-Wide -- Identification of Translation Start Sites in Bacterial Genomes -- Genome-Wide Ribosome Profiling of the Plasmodium falciparum Intraerythrocytic Developmental Cycle -- Performing Ribosome Profiling to Assess Translation in Vegetative and Meiotic Yeast Cells -- Quantitative Comparisons of Translation Activity by Ribosome Profiling with Internal Standards -- Genome-Wide Analysis of Translation in Replicatively Aged Yeast -- Tissue-Specific Ribosome Profiling in Drosophila -- Measuring Organ-Specific Translation Elongation Rate in Mice -- Active Ribosome Profiling with RiboLace: From Bench to Data Analysis -- Poly-A Tailing and Adaptor Ligation Methods for Ribo-Seq Library Construction -- Choice of Ribonucleases for Ribosome Profiling Experiments -- Codon Resolution Analysis of Ribosome Profiling Data -- Assessing Ribosome Distribution Along Transcripts with Polarity Scores and Regression Slope Estimates -- Unsupervised Bayesian Prediction of RNA Translation from Ribosome Profiling Data -- uORF-seqr: A Machine Learning-Based Approach to the Identification of Upstream Open Reading Frames in Yeast -- Genome-Wide Analysis of Actively Translated Open Reading Frames Using RiboTaper/ORFquant.
520 _aThis book provides a single-source reference on the current state of the ribosome profiling method by describing experimental protocols for the quantitative analysis of translation in a variety of model organisms. In addition, the volume presents a detailed overview of the existing software tools and includes detailed description of methods for statistical analysis, data processing, and visualization of ribosome profiling data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ribosome Profiling: Methods and Protocols aims to provide the type of standardized protocols that have previously been unavailable in an effort to bypass the major barriers to wide use of ribosome profiling-based approaches.
700 1 _aLabunskyy, Vyacheslav M
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071611494
776 0 8 _iPrinted edition:
_z9781071611517
776 0 8 _iPrinted edition:
_z9781071611524
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1150-0
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2021
999 _c233122
_d233122