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020 _a9781071613023
024 7 _a10.1007/978-1-0716-1302-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aHelicobacter Pylori
_cedited by Sinead M Smith.
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XI, 234 páginas)
_b38 ilustraciones, 30 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2283
505 0 _a An Overview of Helicobacter pylori Infection -- The Urea Breath Test for the Non-Invasive Detection of Helicobacter pylori -- Real-time Diagnosis of Helicobacter pylori during Endoscopy by Gastric Juice Analysis -- Molecular Detection of Antibiotic-resistant Helicobacter pylori -- Endoscopic Detection of Helicobacter pylori by the Rapid Urease Test -- Culture-based Antimicrobial Susceptibility Testing for Helicobacter pylori -- Purification of Total RNA from Stomach Tissue Biopsies -- Gene Expression Analysis by Reverse Transcription Quantitative PCR -- A Method to Investigate the Helicobacter pylori-associated DNA Methylome -- Profiling the Gastrointestinal Microbiota -- Helicobacter pylori Virulence Factor Genotyping -- Generation of Isogenic Mutant Strains of Helicobacter pylori -- Isolation of Outer Membrane Vesicles from Helicobacter pylori -- A Mouse Model of Helicobacter pylori Infection -- Investigating the Interaction of Helicobacter pylori with the Gastric Mucosa -- Evaluating Host Responses to Helicobacter pylori using ELISA and Western Blot -- Analysis of Innate Immune Responses to Helicobacter pylori -- Analysis of T Cell Responses to Helicobacter pylori Infection -- Miniaturised In Vitro Assays to Study Cellular Phenotypic Characteristics: Proliferation, Migration, Invasion, and Anoikis-resistance.
520 _aThis book brings together a collection of methods for the successful detection, culture and investigation of H. pylori, and its associated diseases. Chapters guide the reader through overviews on H. pylori infection, protocols for analyzing human clinical samples, and protocols for characterizing H. pylori, its functional components and its interactions with host cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Helicobacter pylori: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.
700 1 _aSmith, Sinead M
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071613016
776 0 8 _iPrinted edition:
_z9781071613030
776 0 8 _iPrinted edition:
_z9781071613047
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1302-3
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2021
999 _c233123
_d233123