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008			210330s2021 xxu| s |||| 0|eng d
020			_a9781071612170
024	7		_a10.1007/978-1-0716-1217-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCell Cycle Checkpoints _bMethods and Protocols _cedited by James J. Manfredi.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 242 páginas) _b31 ilustraciones, 21 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2267
505	0		_aDetermination of CHK1 Cellular Localization by Immunofluorescence Microscopy -- Detection of Post-Translationally Modified p53 by Western Blotting -- Global Analyses to Identify Direct Transcriptional Targets of p53 -- Analysis of Replication Dynamics Using the Single-Molecule DNA Fiber Spreading Assay -- Measuring Translation Efficiency by RNA Immunoprecipitation of Translation Initiation Factors -- DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nucleic Acids -- A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer -- The DNA Damage Response in Xenopus laevis Cell-Free Extracts -- Mammalian Cell Fusion Assays for the Study of Cell Cycle Progression by Functional Complementation -- Knockdown of Target Genes by siRNA In Vitro -- Assaying Cell Cycle Status Using Flow Cytometry -- Using TUNEL Assay to Quantitate p53-Induced Apoptosis in Mouse Tissues -- Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast -- Calreticulin Exposure in Mitotic Catastrophe -- Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy -- Clonogenic Assays to Detect Cell Fate in Mitotic Catastrophe.
520			_aThis volume explores the latest advancements in the field of cell cycle checkpoints and their implications for human diseases. Chapters in this book cover topics such as post-translationally modified p53 by western blotting; CHK1 cellular localization by immunofluorescence microscopy; DNA affinity purification; knockdown of target genes by siRNA in vitro; and calreticulin exposure in mitotic catastrophe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cell Cycle Checkpoints: Methods and Protocols is a valuable resource for researcher interested in learning more about this developing field.
700	1		_aManfredi, James J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071612163
776	0	8	_iPrinted edition: _z9781071612187
776	0	8	_iPrinted edition: _z9781071612194
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1217-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233124 _d233124