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008			210706s2021 xxu| s |||| 0|eng d
020			_a9781071614419
024	7		_a10.1007/978-1-0716-1441-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMammalian Cell Engineering _bMethods and Protocols _cedited by Ryosuke Kojima.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 330 páginas) _b83 ilustraciones, 67 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2312
505	0		_aGeneration of CAR-T Cells by Lentiviral Transduction -- Synthetic Receptors for Sensing Soluble Molecules with Mammalian Cells -- Engineering Mammalian Cells to Control Glucose Homeostasis -- Using Engineered Mammalian Cells for an Epitope-Directed Antibody Affinity Maturation System -- Purification of Specific Cell Populations Differentiated from Stem Cells using MicroRNA-Responsive Synthetic Messenger RNAs -- Green Light-Controlled Gene Switch for Mammalian and Plant Cells -- Sonogenetic Modulation of Cellular Activities in Mammalian Cells -- Constructing Smartphone-Controlled Optogenetic Switches in Mammalian Cells -- Constructing a Smartphone-Controlled Semiautomatic Theranostic System for Glucose Homeostasis in Diabetic Mice -- Construction of Caffeine-Inducible Gene Switches in Mammalian Cells -- Multiplexed Genome Engineering with Cas12a -- Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cells -- Optical Control of Genome Editing by Photoactivatable Cas9 -- Chemogenetic Control of Protein Localization and Mammalian Cell Signaling by SLIPT -- Engineering Hydrogel Production in Mammalian Cells to Synthetically Mimic RNA Granules -- AgDD System: A Chemical Controllable Protein Aggregates in Cells -- Intracellular Unnatural Catalysis Enabled by an Artificial Metalloenzyme -- Feeder-Free Human Induced Pluripotent Stem Cell Culture using a DNA Aptamer-Based Mimic of Basic Fibroblast Growth Factor -- Protocol for de novo Gene Targeting via In Utero Electroporation -- Magnetically Guided Single Cell Virus Stamping. .
520			_aThis volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described methods to engineer mammalian cells to sense biologically relevant inputs, such as cell contacts and soluble proteins. Part Two looks at techniques to engineer mammalian cells to sense artificial inputs, such as light and ultrasound. Part Three provides cutting-edge CRISPR-Cas-based methods to carry out highly multiplexed genome editing and spatiotemporally controlled genome editing. Part Four discusses ways to control and engineer biological events in mammalian cells in combination with chemical compounds and systems. Part Five explores techniques to engineer specific mammalian cells in targeted manners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Mammalian Cell Engineering: Methods and Protocols is a valuable resource that allows scientists to successfully carry out their research, thus ultimately contributing to the future advancement of this field.
700	1		_aKojima, Ryosuke _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071614402
776	0	8	_iPrinted edition: _z9781071614426
776	0	8	_iPrinted edition: _z9781071614433
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1441-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233126 _d233126