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008			210825s2021 xxu| s |||| 0|eng d
020			_a9781071616659
024	7		_a10.1007/978-1-0716-1665-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTargeted Protein Degradation _bMethods and Protocols _cedited by Angela M. Cacace, Christopher M. Hickey, Miklós Békés.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 351 páginas) _b67 ilustraciones, 50 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2365
505	0		_a Protein Ligand Interactions Using Surface Plasmon Resonance -- High-Throughput Detection of Ligand-Protein Binding using a SplitLuc Cellular Thermal Shift Assay -- Proteins and their Interacting Partners: An Introduction to Protein-Ligand Binding Site Prediction Methods with a Focus on FunFOLD3 -- Evaluating ligands for ubiquitin ligases using affinity beads -- Mechanistic and Structural Features of PROTAC Ternary Complexes -- MST and TRIC Technology to Reliably Study PROTAC Binary and Ternary Binding In Drug Development -- An in vitro Pull-down Assay of the E3 ligase:PROTAC:Substrate Ternary Complex to Identify Effective PROTACs -- Kinetic detection of E3: PROTAC: Target Ternary Complexes using NanoBRET technology in Live Cells -- Inducing Ubiquitylation and Protein Degradation as a Drug Development Strategy -- Methodological Versatility of Tandem Ubiquitin Binding Entities (TUBEs) for Understanding Poly-ubiquitination and its Various Chains -- Global Mass Spectrometry-Based Analysis of Protein Ubiquitination Using K-e-GG Remnant Antibody Enrichment -- Determination of Proteasomal Unfolding Ability -- Methods for Quantitative Assessment of Protein Degradation -- A High Throughput method to prioritize PROTAC intracellular target engagement and cell permeability using NanoBRET -- Profiling CELMoD Mediated Degradation of Cereblon Neosubstrates -- Global Proteome Profiling to Assess Changes in Protein Abundance using Isobaric Labeling and Liquid Chromatography Tandem Mass Spectrometry -- PHOTACs Enable Optical Control of Protein Degradation -- Protocols for synthesis of SNIPERs and the methods to evaluate the anti-cancer affects.
520			_aThis volume contains a collection of innovative techniques for studying targeted protein degradation. Chapters guide readers through heterobifunctional proteolysis-targeting chimeras (PROTACs) approaches, E3 ligase, E3 ligase-induced ubiquitylation, proteomic approaches, novel degrader molecules, molecular glue, and stabilize binding interaction between a target and E3 ubiquitin ligase. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Targeted Protein Degradation: Methods and Protocols aims to ensure successful results in this emerging field of drug discovery. .
700	1		_aCacace, Angela M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHickey, Christopher M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBékés, Miklós _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071616642
776	0	8	_iPrinted edition: _z9781071616666
776	0	8	_iPrinted edition: _z9781071616673
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1665-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233136 _d233136