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020 _a9781071616093
024 7 _a10.1007/978-1-0716-1609-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aSolanum tuberosum
_bMethods and Protocols
_cedited by David Dobnik, Kristina Gruden, Živa Ramšak, Anna Coll.
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XI, 418 páginas)
_b105 ilustraciones, 92 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2354
505 0 _aImportance of Potato as a Crop and Practical Approaches to Potato Breeding -- Cryopreservation of Potato Shoot Tips for Long Term Storage -- RNA Sequencing Analyses for Deciphering Potato Molecular Response -- Yeast-2-hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum -- Potato as a Model for Field Trials with Modified Gene Functions in Research And Translational Experiments -- DAP-seq Identification of Transcription Factor Binding Sites in Potato -- Mass Spectrometric Monitoring Of Plant Hormone Crosstalk During Biotic Stress Responses In Potato (Solanum tuberosum L.) -- A Comprehensive Guide to Potato Transcriptome Assembly -- MapMan visualization of RNA-seq data using Mercator4 functional annotations -- Identification of Resistance Genes Using Diagnostic R-gene Enrichment Sequencing (dRenSeq) -- Methodologies for Discovery and Quantitative Profiling of sRNAs in Potato -- Co-expression for Genotype-Phenotype Function Annotation In Potato Research -- Computer Vision and Less Complex Image Analyses to Monitor Potato Traits In Fields -- Quantifying the contribution to virulence of Phytophthora infestans effectors in potato -- Identification of Solanum immune receptors by Bulked Segregant RNA-Seq and high-Throughput Recombinant Screening -- Gene editing in potato using CRISPR-Cas9 technology -- Gene downregulation in potato roots using Agrobacterium rhizogenes-mediated Transformation -- Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato -- Towards the design of potato tolerant to abiotic stress -- Rapid loop-mediated isothermal amplification for detection of the Ralstonia solanacearum species complex bacteria in symptomatic potato tubers and plants. .
520 _aThis volume offers a comprehensive collection of experimental approaches to study Solanum tuberosum (potato). Chapters are divided into five sections covering a general overview of potato, the research fields of molecular biology, omics approaches, and bioinformatics, and finally, the application of the generated knowledge for crop improvement. Written in the format of the highly successful Methods in Molecular Biology series, each wet-lab chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Solanum tuberosum: Methods and Protocols aims to provide a collection of methods for the potato research community that will enable scientists gain insight into the world of the potato. Chapter 2 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
700 1 _aDobnik, David
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aGruden, Kristina
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRamšak, Živa
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aColl, Anna
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071616086
776 0 8 _iPrinted edition:
_z9781071616109
776 0 8 _iPrinted edition:
_z9781071616116
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1609-3
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2021
999 _c233137
_d233137