000			03892nam a22003735i 4500
001			233148
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007			cr nn 008mamaa
008			100301s1997 xxu| s |||| 0|eng d
020			_a9781592595921
024	7		_a10.1385/0896033813 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHelicobacter pylori Protocols _cedited by Christopher L. Clayton, Harry L. T. Mobley.
250			_a1st edition 1997
264		1	_aTotowa, NJ _bHumana Press _c1997
300			_a1 recurso en línea (XIII, 274 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v8
505	0		_aIntroduction: Medical Significance of H. pylori -- Detection of H. pylori Infection by Biopsy Urease, Histology, and Culture -- Serology and Urea Breath Test in the Diagnosis of H. pylori Infection -- The Detection of H. pylori by the Polymerase Chain Reaction -- PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples -- In vitro Susceptibility Testing of H. pylori -- Defined Media for H. pylori -- Studies of Respiration in H. pylori -- Use of NMR to Study H. pylori Metabolism -- Isolation of H. pylori Genomic DNA and Restriction Analysis -- Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of H. pylori -- Differentiation of H. pylori Strains Using PCR RFLP -- PCR-DNA Sequence Typing -- H. pylori DNA Fingerprinting Using the Arbitrarily Primed PCR (AP-PCR) or Random Amplified Polymorphic DNA (RAPD) Method -- Molecular Methods for Detecting Ulcerogenic Strains of H. pylori -- H. pylori DNA Transformation by Natural Competence and Electroporation -- Random Mutagenesis of the H. pylori Genome -- Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE) -- Molecular Characterization of H. pylori Surface Antigens -- Isolation and Characterization of Porins from H. pylori -- Methods for the Identification of H. pylori Host Receptors -- Host Responses to H. pylori -- Nonprimate Animal Models of H. pylori Infection -- The Nonhuman Primate Model for H. pylori Infection.
520			_aIn Helicobacter pylori Protocols, leading experts bring together an outstanding collection state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for culturing H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed to solve the difficult technical problems created by the application of today's powerful bacterial genetic and molecular cloning techniques to H. pylori. Helicobacter pylori Protocols contains a wide variety of stand-alone techniques that are unfailingly reproducible. The book is certain to become a standard experimental and clinical laboratory reference for all those engaged in H. pylori work today.
700	1		_aClayton, Christopher L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMobley, Harry L. T _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617370205
776	0	8	_iPrinted edition: _z9780896033818
776	0	8	_iPrinted edition: _z9781489927965
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033813 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1997
999			_c233148 _d233148