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001			233165
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007			cr nn 008mamaa
008			180331s2018 xxu| s |||| 0|eng d
020			_a9781493977680
024	7		_a10.1007/978-1-4939-7768-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCpG Islands _bMethods and Protocols _cedited by Tanya Vavouri, Miguel A. Peinado.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 340 páginas) _b52 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1766
505	0		_aCpG Islands: A Historical Perspective -- Biochemical Identification of Non-Methylated DNA by BioCAP-Seq -- Prediction of CpG Islands as an Intrinsic Clustering Property Found in Many Eukaryotic DNA Sequences and Its Relation to DNA Methylation -- CpG Islands in Cancer: Heads, Tails, and Sides.- Infinium DNA Methylation Microarrays on Formalin-Fixed Paraffin-Embedded Samples.- The Use of Methylation-Sensitive Multiplex Ligation-Dependent Probe Amplification for Quantification of Imprinted Methylation -- The Pancancer DNA Methylation Trackhub: A Window to The Cancer Genome Atlas Epigenomics Data -- Methylation-Sensitive Amplification Length Polymorphism (MS-AFLP) Microarrays for Epigenetic Analysis of Human Genomes -- Genome-Wide Profiling of DNA Methyltransferases in Mammalian Cells -- Experimental Design and Bioinformatic Analysis of DNA Methylation Data -- Assay for Transposase Accessible Chromatin (ATAC-Seq) to Chart the Open Chromatin Landscape of Human Pancreatic Islets -- Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-Seq) -- Genome-Wide Mapping of Protein-DNA Interactions on Nascent Chromatin -- Analysis of Chromatin Interactions Mediated by Specific Architectural Proteins in Drosophila Cells -- High-Throughput Single-Cell RNA Sequencing and Data Analysis -- Functional Insulator Scanning of CpG Islands to Identify Regulatory Regions of Promoters Using CRISPR -- An Application-Directed, Versatile DNA FISH Platform for Research and Diagnostics.
520			_aThis detailed volume examines bioinformatic and molecular biological methods useful to identify and to explore the functions of CpG islands, key navigation points to understand gene regulation in fundamental processes such as development and cell differentiation as well as in diseases like cancer. Beginning with a historical perspective and important properties of CpG islands, the book continues with sections on computational and wet lab methods related to the study of DNA methylation, and in-depth protocols for the analysis of CpG island functional features including epigenetic profiling and chromatin interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, CpG Islands: Methods and Protocols aims to provide readers with the information and methodologies necessary to continue to decipher how a genome's structure and organization contribute to regulate biological processes.
700	1		_aVavouri, Tanya _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPeinado, Miguel A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493977673
776	0	8	_iPrinted edition: _z9781493977697
776	0	8	_iPrinted edition: _z9781493992799
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7768-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233165 _d233165