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020 _a9781493978748
024 7 _a10.1007/978-1-4939-7874-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Chemical Genomics
_bMethods and Protocols
_cedited by Friedrich Fauser, Martin Jonikas.
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XI, 242 páginas)
_b60 ilustraciones, 47 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1795
505 0 _aMulti-Parametric Screening in Arabidopsis thaliana Seedlings -- Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress -- Method for Ultra-Rapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen -- A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death using Suspension Cell Culture of Arabidopsis thaliana -- High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression -- Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana using Chemical Genetics -- Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls using BY-2 Cells -- Small Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes -- High-Throughput In Vitro Screening for Inhibitors of Cereal α-glucosidase -- Chemical Screening for Strigolactone Receptor Antagonists using Arabidopsis thaliana -- Chemical Control of ABA Receptors to Enable Plant Protection against Water Stress -- Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening -- Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside that Controls the Leaf Closure of Samanea saman -- Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule -- Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants using PlantiSMASH -- Chemical Genomics Translatability from Unicellular to Multicellular Models -- Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii -- FnCpf1-Mediated Targeted Mutagenesis in Plants.
520 _aThis volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.
700 1 _aFauser, Friedrich
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aJonikas, Martin
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493978731
776 0 8 _iPrinted edition:
_z9781493978755
776 0 8 _iPrinted edition:
_z9781493993116
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7874-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c233166
_d233166