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020 _a9781493987429
024 7 _a10.1007/978-1-4939-8742-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMicrobial Carotenoids
_bMethods and Protocols
_cedited by Carlos Barreiro, José-Luis Barredo.
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XII, 343 páginas)
_b58 ilustraciones, 21 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1852
505 0 _aAdvancement of Biotechnology by Genetic Modifications -- Carotenoids Production: A Healthy and Profitable Industry -- Carotenoids: From Plants to Food and Feed Industries -- Express Analysis of Microalgal Secondary Carotenoids by TLC and UV-Vis Spectroscopy -- Batch Cultivation for Astaxanthin Analysis Using the Green Microalga Chlorella zofingiensis under Multitrophic Growth Conditions -- Preparative Recovery of Carotenoids from Microalgal Biomass -- Adaptive Laboratory Evolution for Enhanced Carotenoid Production in Microalgae -- Carotenoid Production by Recombinant Corynebacterium glutamicum: Strain Construction, Cultivation, Extraction and Quantification of Carotenoids and Terpenes -- Rapid and Selective Screening Method for Isolation and Identification of Carotenoid-producing Bacteria -- Purification and Identification of Astaxanthin and its Novel Derivative Produced by Radio-tolerant Sphingomonas astaxanthinifaciens -- Screening, Isolation, and Identification of Zeaxanthin-producing Bacteria -- Synthesis of Carotenoids of Industrial Interest in the Photosynthetic Bacterium Rhodopseudomonas palustris: Bioengineering and Growth Conditions -- Molecular Tools for Carotenogenesis Analysis in the Mucoral Mucor circinelloides -- Expression Vectors and Gene Fusions for the Directed Modification of the Carotenoid Biosynthesis Pathway in Mucor circinelloides -- Lycopene Production by Mated Fermentation of Blakeslea trispora -- HPLC Analysis of Carotenoids in Neurosporaxanthin-producing Fungi -- Extraction and Analysis of Carotenes and Xanthophylls Produced by Xanthophyllomyces dendrorhous -- Isolation and Selection of New Astaxanthin Producing Strains of Phaffia rhodozyma -- Engineering Pichia pastoris for the Production of Carotenoids -- Isolation and Characterization of Extrachromosomal Double-Stranded RNA Elements from Carotenogenic Yeasts.
520 _aIn this volume, expert researchers in the field detail the most up-to-date methods commonly used to study and produce carotenoids. These include methods on the manipulation and metabolic engineering of carotenoid producing microalgae and bacteria, including Corynebacterium glutamicum, Rhodopseudomonas palustris and radio-tolerant bacteria; in addition to fungi, as the beta-carotene producing Blakeslea trispora and Mucor circinelloides or the lycopene producing Blakeslea trispora; and the heterobasidiomycetous yeast producing xanthophylls Xanthophyllomyces dendrorhous (Phaffia rhodozyma) and the engineered yeast Pichia pastoris. Additionally, three overview chapters on the advancement of Biotechnology and carotenoid production are included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microbial Carotenoids: Methods and Protocols provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms, aiming to ensure successful results in the further study of this vital field.
700 1 _aBarreiro, Carlos
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBarredo, José-Luis
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493987412
776 0 8 _iPrinted edition:
_z9781493987436
776 0 8 _iPrinted edition:
_z9781493993802
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8742-9
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c233173
_d233173