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001			233177
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007			cr nn 008mamaa
008			180827s2018 xxu| s |||| 0|eng d
020			_a9781493987849
024	7		_a10.1007/978-1-4939-8784-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aXenopus _bMethods and Protocols _cedited by Kris Vleminckx.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 302 páginas) _b68 ilustraciones, 57 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1865
505	0		_aHusbandry, General Care, and Transportation of Xenopus laevis, and Xenopus tropicalis -- Generation and Care of Xenopus laevis and Xenopus tropcalis Embryos -- Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering -- Targeted Genome Engineering in Xenopus using the Transcription activator-Like Effector Nuclease (TALEN) Technology -- Genotyping of CRISPR/Cas9 Genome Edited Xenopus tropicalis -- BATCH-GE: Analysis of NGS Data for Genome Editing Assessment -- A Simple Knock-In System for Xenopus via Microhomology-Mediated End Jointing Repair -- How to Generate Non-Mosaic CRSPR/Cas9 Mediated Knock-In and Mutations F0 Xenopus through the Host-Transfer Technique -- Targeted Electroporation in the CNS in Xenopus Embryos -- Conditional Chemogenetic Ablation of Photoreceptor Cells in Xenopus retina -- Cancer Models in Xenopus tropicalis by CRISPR/Cas 9 Mediated Knockout of Tumor Suppressors -- CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis -- Quantitative Proteomics of Xenopus Embryos I, Sample Preparation -- Quantitative Proteomics for Xenopus Embryos II, Data Analysis -- Dye Electroporation and Imaging of Calcium Signaling in Xenopus Nervous System -- X-FaCT: Xenopus-Fast Clearing Technique -- Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow Cytometry -- Manipulating and Analyzing Cell Type Composition of the Xenopus Mucociliary Epidermis -- Evaluating Blood Cell Populations in Xenopus using Flow Cytometry and Differential Counts by Cytospin -- Isolation and Culture of Amphibian (Xenopus laevis) Sub-Capsular Liver and Bone Marrow Cells -- Isolation and Primary Culture Methods of Adult and Larval Myogenic Cells from Xenopus laevis.
520			_aThis volume explores techniques used to study and experiment with Xenopus in order to model or understand human disease. The chapters in this book cover topics such as implementation of CRISPR/Cas9 and TALEN in Xenopus from the design stage up to the genotyping stage; methods to explore generation of knock-in animals; cancer modelling and in vivo screening of congenital heart disease; electroporation; and phenotyping at the organismal level, cellular level, and proteome level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Xenopus: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this wide and developing field. This book will aid in keeping Xenopus at the forefront for biochemical, cell biological, and developmental studies and forward it as a preclinical disease model.
700	1		_aVleminckx, Kris _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493987832
776	0	8	_iPrinted edition: _z9781493987856
776	0	8	_iPrinted edition: _z9781493993918
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8784-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233177 _d233177