000			03558nam a22003615i 4500
001			233183
003			ES-VaUE
005			20221220020443.0
007			cr nn 008mamaa
008			180907s2018 xxu| s |||| 0|eng d
020			_a9781493986453
024	7		_a10.1007/978-1-4939-8645-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSerpins _bMethods and Protocols _cedited by Alexandra Lucas.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIII, 276 páginas) _b45 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1826
505	0		_aOverview of Serpins and Their Role in Biological Systems -- Methods for Determining and Understanding Serpin Structure and Function: X-Ray Crystallography -- Serpin Phage Display: Use of a T7 System to Probe Reactive Centre Loop Libraries with Different Serine Proteinases -- Kinetic Measurement of Serpin Inhibitory Activity by Real-Time Fluorogenic Biochemical Assay -- Methods for Identifying Virus-Derived Serpins -- In Vitro Approaches for the Assessment of Serpin Polymerization -- Cellular Models for the Serpinopathies -- Binding of Serpins to Immobilized Phospholipids and Phospholipids in Suspension -- Viral Serpin Reactive Center Loop (RCL) Peptides: Design and Testing -- In Vivo Analysis of Alpha 1 Antitrypsin Functions in Autoimmune Disease Models -- Analysis of In Vivo Serpin Functions in Models of Inflammatory Vascular Disease -- In Vivo Analysis of Alpha 1 Antitrypsin Functions in Autoimmune Disease Models -- Serpins in Venous Thrombosis and Venous Thrombus Resolution -- Next Generation Sequencing Library Preparation for 16S rRNA Microbiome Analysis after Serpin Treatment -- Methods for Assessing Serpins as Neuroprotective Therapeutics -- Serpins: Development as Clinical Therapeutics -- Serine Protease Assays: Measuring the Enzyme Targets for Serpins, Serine Protease Inhibitors. .
520			_aThis volume explores the latest methods used to study and define serpin molecular structure, basic protease inhibition, serpin targets, and the roles of serpin in biology and disease using animal models. The chapters in this book cover topics such as crystallography and phage display, peptide design, phospholipid binding, and thrombus formation to microbiome analysis and development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Serpins: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this evolving field.
700	1		_aLucas, Alexandra _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493986446
776	0	8	_iPrinted edition: _z9781493986460
776	0	8	_iPrinted edition: _z9781493993529
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8645-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233183 _d233183