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020 _a9781493986972
024 7 _a10.1007/978-1-4939-8697-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aSomatic Stem Cells
_bMethods and Protocols
_cedited by Shree Ram Singh, Pranela Rameshwar.
250 _a2nd edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XI, 284 páginas)
_b58 ilustraciones, 52 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1842
505 0 _aAn Update on the Therapeutic Potential of Stem Cell -- Single-Step Plasmid Based Reprogramming of Human Dermal Fibroblasts to Induced Neural Stem Cells -- Isolation and Analysis of Mesenchymal Progenitors of the Adult Hematopoietic Niche -- Identification and Isolation of Mice and Human Hematopoietic Stem Cells -- Identification and Characterization of Hair Follicle Stem Cells -- Methods of Mesenchymal Stem Cell Homing to the Blood-Brain Barrier -- 3D Bioprinting and Stem Cells -- Characterization of Gastrospheres Using 3D Coculture System -- Markers and Methods to Study Intestinal Stem Cells -- Quantitative Analysis of Intestinal Stem Cell Dynamics using Microfabricated Cell Culture Arrays -- Detection, Labeling, and Culture of Lung Stem and Progenitor Cells -- Isolation, Characterization, and Differentiation of Mouse Cardiac Progenitor Cells -- Isolating and Characterizing Adipose-Derived Stem Cells -- Enzyme-Free Isolation of Adipose-Derived Mesenchymal Stem Cell -- Identification and Characterizations of Annulus Fibrosus-Derived Stem Cells -- The Maintenance of Tendon Stem/Progenitor Cells in Culture -- Intravital Imaging to Understand Spatiotemporal Regulation of Osteogenesis and Angiogenesis in Cranial Defect Repair and Regeneration -- Beating Heart Cells from Hair Cells -- Generation of FLIP and FLIP-FlpE Targeting Vectors for Bi-Allelic Conditional and Reversible Gene Knockouts in Mouse and Human Cells -- Analytical Platforms and Techniques to Study Stem Cells Metabolism.
520 _aThis second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a variety of organic tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Somatic Stem Cells: Methods and Protocols, Second Edition is a valuable resource for both novice and experienced molecular biologists, developmental biologists, tissue engineers, and geneticists who are interested in stem cell research and its potentials in regenerative medicine.
700 1 _aSingh, Shree Ram
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRameshwar, Pranela
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493986965
776 0 8 _iPrinted edition:
_z9781493986989
776 0 8 _iPrinted edition:
_z9781493993697
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8697-2
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c233185
_d233185